5(6)-FITC (Fluorescein 5(6)-isothiocyanate), Fluorescent amine-reactive label (ab145258)
Key features and details
- Green fluorescent, amine-reactive label
- CAS Number: 27072-45-3
- Purity: > 90%
- Soluble in ethanol to 50 mM and in DMSO to 10 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
5(6)-FITC (Fluorescein 5(6)-isothiocyanate), Fluorescent amine-reactive label -
Description
Green fluorescent, amine-reactive label -
Biological description
Green fluorescent, amine-reactive label. Efficiently labels antibodies and other purified proteins at primary amines (lysine side chains). Used in applications including fluorescence microscopy, flow cytometry western blotting and ELISA. 5(6)-FITC DA (ab145463) precursor. 5-FITC (ab145325) and 6-FITC (ab146082) isomer mixture.
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Purity
> 90% -
CAS Number
27072-45-3 -
Chemical structure
Properties
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Excitation
492nm -
Emission
518nm -
Chemical name
Fluorescein 5(6)-isothiocyanate -
Molecular weight
389.38 -
Molecular formula
C21H11NO5S -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in ethanol to 50 mM and in DMSO to 10 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic
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Research areas
