HRP Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab197905)
![HRP Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab197905)](https://www.abcam.com/ps/products/197/ab197905/Images/ab197905-242605-WBab1979051.jpg "HRP Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab197905)")
Key features and details
- HRP Mouse monoclonal [3D5] to ATPB - Mitochondrial Marker
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-ATPB antibody [3D5] - Mitochondrial Marker
See all ATPB primary antibodies -
Description
HRP Mouse monoclonal [3D5] to ATPB - Mitochondrial Marker -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Tissue, cells or virus corresponding to Human ATPB.
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Positive control
- WB: Human heart mitochondrial lysate. HeLa and HepG2 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
3D5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - HRP Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab197905)All lanes : HRP Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab197905) at 1/5000 dilution
Lane 1 : Human heart tissue lysate - mitochondrial extract (ab110337) at 5 µg
Lane 2 :HeLa whole cell lysate (ab150035) at 10 µg
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 2 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197905 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
