Anti-Heparanase 1 antibody (ab85543)
Key features and details
- Rabbit polyclonal to Heparanase 1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, Chinese hamster
- Isotype: IgG
Overview
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Product name
Anti-Heparanase 1 antibody
See all Heparanase 1 primary antibodies -
Description
Rabbit polyclonal to Heparanase 1 -
Host species
Rabbit -
Specificity
ab85543 recognizes the 65kD precursor as well as the 50kD and 8kD subunits of Heparanase 1. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human, Chinese hamster -
Immunogen
Human 50kD-8kD Heparanase 1 heterodimer.
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Positive control
- Spleen tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heparanase 1 antibody (ab85543) This image is courtesy of an Abreview submitted by Jim Manavisab85543 staining Heparanase 1 in Human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% NHS for 30 minutes at room temperature; antigen retrieval was by heat mediation with an EDTA buffer. Samples were incubated with primary antibody (1/4000 in NHS) for 8 hours. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
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Western blot - Anti-Heparanase 1 antibody (ab85543)Purified recombinant human heparanase served as a control (Lane1). Lane2: An extract from 6.5x10e3 CHO cells, transfected with human Heparanase; Lane3: extract from 8x10e4 CHO cells, transfected with mouse Heparanase, and Lane4: extract from 5x10e5 non-transfected CHO dhfr- cells, were loaded onto 4-12% NuPage gel. The proteins were transferred to a PVDF and subjected to Western Blot analysis using ab85543 at 1:1000 dilution. The 65kD precursor and the 50kD and 8kD subunits are clearly detected. Lane 1 : Purified recombinant human Heparanase served as a control
Lane 2 : Extract from 6.5x10e3 CHO cells, transfected with human Heparanase
Lane 3 : Extract from 8x10e4 CHO cells, transfected with mouse Heparanase
Lane 4 : Extract from 5x10e5 non-transfected CHO dhfr- cells
Cell were loaded onto 4-12% NuPage gel. The proteins were transferred to a PVDF and subjected to Western Blot analysis using ab85543 at 1:1000 dilution. The cleavage of the 65 kDa form leads -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heparanase 1 antibody (ab85543)ab85543, at 5µg/ml, staining Heparanase 1 in formalin-fixed, paraffin-embedded human spleen tissue by Immunohistochemistry.
