Anti-Heparanase 1 antibody (ab232817)
Key features and details
- Rabbit polyclonal to Heparanase 1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Heparanase 1 antibody
See all Heparanase 1 primary antibodies -
Description
Rabbit polyclonal to Heparanase 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment (His-tag) corresponding to Human Heparanase 1 aa 1-150. (Expressed in E.coli).
Database link: Q9Y251 -
Positive control
- IHC-P: Human spleen tissue. WB: HeLa and HepG2 cell lysates; Recombinant human Heparanase 1 protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab232817 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heparanase 1 antibody (ab232817)
Formalin-fixed, paraffin-embedded human spleen tissue stained for Heparanase 1 using ab232817 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Western blot - Anti-Heparanase 1 antibody (ab232817)Anti-Heparanase 1 antibody (ab232817) at 3 µg/ml + Recombinant human Heparanase 1 protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 61 kDa
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Western blot - Anti-Heparanase 1 antibody (ab232817)All lanes : Anti-Heparanase 1 antibody (ab232817) at 3 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 3 : Human liver lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 61 kDa
