Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human Chk2 Antibody Pair - BSA and Azide free (ab243996)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

Overview

  • Product name

    Human Chk2 Antibody Pair - BSA and Azide free
    See all Chk2 kits

  • Assay type

    ELISA set

  • Range

    0.23 ng/ml - 15 ng/ml

  • Species reactivity

    Reacts with: Human

  • Product overview

    The Antibody Pair can be used to quantify Human Chk2. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab218271), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.

  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human Chk2 Capture Antibody (unconjugated) 1 x 100µg
    Human Chk2 Detector Antibody (unconjugated) 1 x 100µg

  • Research areas

  • Function

    Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.

  • Tissue specificity

    High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.

  • Involvement in disease

    Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
    Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
    Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500].

  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
    Contains 1 FHA domain.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Phosphorylated by PLK4.

  • Cellular localization

    Nucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53.
  • Information by UniProt

  • Alternative names

    • CDS 1
    • Cds1
    • Cds1 homolog
    • Checkpoint kinase 2
    • Checkpoint like protein CHK2
    • CHEK 2
    • Chek2
    • Chk 2
    • CHK2 checkpoint homolog
    • CHK2 checkpoint homolog (S. pombe)
    • CHK2_HUMAN
    • hCds1
    • HuCds 1
    • LFS 2
    • LFS2
    • PP1425
    • RAD 53
    • RAD53
    • Rad53 homolog
    • Serine/threonine protein kinase Chk2
    • Serine/threonine-protein kinase Chk2
    see all

  • Database links

    Images

    • Sandwich ELISA - Human Chk2 Antibody Pair - BSA and Azide free (ab243996)
      Sandwich ELISA - Human Chk2 Antibody Pair - BSA and Azide free (ab243996)
      To learn more about the advantages of recombinant antibodies see here.

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