Anti-Histone H3 (acetyl K36) antibody (ab272159)
Key features and details
- Rabbit polyclonal to Histone H3 (acetyl K36)
- Suitable for: WB, Dot blot
- Reacts with: Human, Caenorhabditis elegans
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (acetyl K36) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (acetyl K36) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IF HumanWB Caenorhabditis elegans
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Immunogen
Synthetic peptide corresponding to Human Histone H3 (acetyl K36).
Database link: Q71DI3 -
Positive control
- WB: Caenorhabditis elegans embryo lysate. IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.0004% Potassium phosphate, 0.0008% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunofluorescence - Anti-Histone H3 (acetyl K36) antibody (ab272159)
Immunofluorescence analysis of ab272159 was tested at 1:200,000 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells with FITC (green). Cells and nuclei were counterstained with DAPI (blue) and DyLight 550 (red). (40X)
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Western blot - Anti-Histone H3 antibody (ab272159)Caenorhabditis elegans embryo lysate at 30 µg
Predicted band size: 15 kDaBlock: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~15 kDa.
Other band(s): None.
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Dot Blot - Anti-Histone H3 antibody (ab272159)Dot Blot analysis of ab272159.
Lane 1: K36.
Lane 2: K36-KMe2.
Lane 3: K36-KAc.
Lane 4: K36-KMe1.
Load: 0.03, 0.1, 0.3, 1.0, 3.0 µg of peptide. Primary antibody ab272159 was used overnight at 4°C. HRP rabbit secondary antibody was used at 1:10,000 for 45 minutes at room temperature.
Block: 5% BLOTTO A overnight at 4°C.