Anti-Histone H3 (mono methyl K56) antibody (ab272161)
Key features and details
- Rabbit polyclonal to Histone H3 (mono methyl K56)
- Suitable for: WB, Dot blot
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (mono methyl K56) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (mono methyl K56) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IF HumanWB Mouse
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Immunogen
Synthetic peptide corresponding to Human Histone H3 (mono methyl L56).
Database link: Q71DI3 -
Positive control
- WB: NIH/3T3 IF: HeLa
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.0004% Potassium phosphate, 0.0008% Sodium chloride, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Dot Blot - Anti-Histone H3 antibody (ab272161)
Dot Blot analysis of ab272161.
Lane 1: K56Ac.
Lane 2: K56Me1.
Lane 3: K56Me2.
Lane 4: K56Me3.
Lane 5: K56 unmodified.
Load: 1, 10, and 100 picomoles of peptide. Primary antibody ab272161 was at 1:20-1:100 for 45 minutes at 4°C. Dylight™488 rabbit secondary antibody at 1:10,000 for 45 minnutes at room temperature. Block: 5% BLOTTO overnight at 4°C.
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Immunofluorescence - Anti-Histone H3 (mono methyl K56) antibody (ab272161)Immunofluorescence analysis of ab272161. HeLa cells were treated by a primary antibody ab272161 at a 1:100 dilution for 1 hour at room temperature. It was followed by a secondary antibody Dylight 488 at 1:10,000 for 45 minutes at room temprature. Histone H3 [Monomethyl Lys56] localization is nuclear and chromosomal. Histone H3 [Monomethyl Lys56] staining was expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red). Fixation was 0.5% PFA and antigen retrieval was not required. -
Western blot - Anti-Histone H3 antibody (ab272161)NIH/3T3 cell lysate at 30 µg
Secondary
IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.
Predicted band size: 15 kDaBlock: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~15 kDa. Other band(s): None.