Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Wednesday March 03, 2021

Anti-HNF-1B antibody [EPR18644-13] (ab213149)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18644-13] to HNF-1B
Suitable for: IHC-P
Reacts with: Human

Product name

Anti-HNF-1B antibody [EPR18644-13]
See all HNF-1B primary antibodies
Description

Rabbit monoclonal [EPR18644-13] to HNF-1B
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human liver,liver bile duct carcinoma and ovarian clear cell carcinoma tissues.
General notes
Previously labelled as HNF1 beta.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18644-13
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] (ab213149)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling HNF-1B with ab213149 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Nuclear staining on human liver bile duct is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] (ab213149)

Immunohistochemical analysis of paraffin-embedded human liver bile duct carcinoma tissue labeling HNF-1B with ab213149 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Nuclear staining on human liver bile duct carcinoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] (ab213149)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling HNF-1B with ab213149 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Negative staining on human hepatocellular carcinoma. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] (ab213149)

Immunohistochemical analysis of paraffin-embedded human ovarian clear cell carcinoma tissue labeling HNF-1B with ab213149 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Nuclear staining on human ovarian clear cell carcinoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] (ab213149)

Immunohistochemical analysis of paraffin-embedded human serous ovarian carcinoma tissue labeling HNF-1B with ab213149 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Negative staining on human serous ovarian carcinoma. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] (ab213149)

Immunohistochemical analysis of paraffin-embedded human endometrioid ovary adenocarcinoma tissue labeling HNF-1B with ab213149 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Negative staining on human endometrioid ovary adenocarcinoma. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-HNF-1B antibody [EPR18644-13] (ab213149)

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