Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HNF-1B antibody [EPR18644-13] (ab213149)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18644-13] to HNF-1B
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-HNF-1B antibody [EPR18644-13]
    See all HNF-1B primary antibodies

  • Description

    Rabbit monoclonal [EPR18644-13] to HNF-1B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human liver,liver bile duct carcinoma and ovarian clear cell carcinoma tissues.

  • General notes

     

     

    Previously labelled as HNF1 beta. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18644-13

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] (ab213149)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] (ab213149)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling HNF-1B with ab213149 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Nuclear staining on human liver bile duct is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] (ab213149)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] (ab213149)

    Immunohistochemical analysis of paraffin-embedded human liver bile duct carcinoma tissue labeling HNF-1B with ab213149 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Nuclear staining on human liver bile duct carcinoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] (ab213149)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] (ab213149)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling HNF-1B with ab213149 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Negative staining on human hepatocellular carcinoma. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] (ab213149)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] (ab213149)

    Immunohistochemical analysis of paraffin-embedded human ovarian clear cell carcinoma tissue labeling HNF-1B with ab213149 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Nuclear staining on human ovarian clear cell carcinoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] (ab213149)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] (ab213149)

    Immunohistochemical analysis of paraffin-embedded human serous ovarian carcinoma tissue labeling HNF-1B with ab213149 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Negative staining on human serous ovarian carcinoma. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] (ab213149)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] (ab213149)

    Immunohistochemical analysis of paraffin-embedded human endometrioid ovary adenocarcinoma tissue labeling HNF-1B with ab213149 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Negative staining on human endometrioid ovary adenocarcinoma. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-HNF-1B antibody [EPR18644-13] (ab213149)
    Anti-HNF-1B antibody [EPR18644-13] (ab213149)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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