Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Mouse monoclonal [2B3] to RUNX3
Suitable for: WB, IHC-P, Flow Cyt
Knockout validated
Reacts with: Mouse, Human
Isotype: IgG2b

Product name

Anti-RUNX3 antibody [2B3]
See all RUNX3 primary antibodies
Description

Mouse monoclonal [2B3] to RUNX3
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment, corresponding to amino acids 186-252 of Human RUNX3 expressed in E. Coli.
Positive control
- Human RUNX3 recombinant protein; HEK293 cell lysate transfected with RUNX3 (aa186-252)-hIgGFc; NIH 3T3 cells; Human cervical cancer tissue.
General notes

This product was changed from ascites to supernatant. Lot no’s high than GR206339-20 are from Tissue Culture Supernatant

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: 99% PBS

0.5% protein stabilizer
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

2B3
Isotype

IgG2b
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX3 antibody [2B3] (ab135248)

Immunohistochemical analysis of Paraffin-embedded Human cervical cancer tissue labelling RUNX3 with ab135248 at 1/200 dilution followed by DAB staining.
Western blot - Anti-RUNX3 antibody [2B3] (ab135248)

Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: RUNX3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)

Lanes 1 - 3: Merged signal (red and green). Green - ab135248 observed at 44 kDa. Red - loading control, ab176560, observed at 50 kDa.

ab135248 was shown to recognize RUNX3 in wild-type HAP1 cells as signal was lost at the expected MW in RUNX3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and RUNX3 knockout samples were subjected to SDS-PAGE. Ab135248 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Flow Cytometry - Anti-RUNX3 antibody [2B3] (ab135248)

Flow cytometric analysis of NIH 3T3 cells labelling RUNX3 with ab135248 at 1/200 dilution (green). Negative control (red).
Western blot - Anti-RUNX3 antibody [2B3] (ab135248)

Anti-RUNX3 antibody [2B3] (ab135248) at 1/500 dilution + Recombinant Human RUNX3 protein

Predicted band size: 44 kDa
Western blot - Anti-RUNX3 antibody [2B3] (ab135248)

All lanes : Anti-RUNX3 antibody [2B3] (ab135248) at 1/500 dilution

Lane 1 : HEK293 cell lysate, non-transfected
Lane 2 : HEK293 cell lysate, transfected with RUNX3 (amino acids 186-252)-hIgGFc

Predicted band size: 44 kDa

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