Rabbit CXCL13 ELISA Kit (BCA-1) (ab273248)
Key features and details
- Sensitivity: 0.35 ng/ml
- Range: 0.328 ng/ml - 80 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rabbit
Overview
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Product name
Rabbit CXCL13 ELISA Kit (BCA-1)
See all BCA1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.35 ng/ml -
Range
0.328 ng/ml - 80 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 82.44 69% - 100% Plasma 74.71 69% - 85% Cell culture media 89.88 67% - 132% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rabbit -
Product overview
Rabbit CXCL13 ELISA Kit (ab273248) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rabbit CXCL13 in serum (Rabbit CXCL13 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Rabbit CXCL13 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Rabbit CXCL13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Rabbit CXCL13 (BCA-1) coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Rabbit CXCL13 (BCA-1) Antibody 2 vials Rabbit CXCL13 (BCA-1) Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5. -
Tissue specificity
Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ANGIE
- ANGIE2
- B cell attracting chemokine 1
see all -
Database links
- Entrez Gene: 100144333 Rabbit
Images
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Rabbit CXCL13 ELISA Kit (BCA-1) standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.