Anti-PPAR gamma antibody (ab209350)
Key features and details
- Rabbit polyclonal to PPAR gamma
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PPAR gamma antibody
See all PPAR gamma primary antibodies -
Description
Rabbit polyclonal to PPAR gamma -
Host species
Rabbit -
Specificity
No significant homology with PPAR alpha or NUC1. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Mouse PPAR gamma aa 250-350.
Database link: P37238
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-PPAR gamma antibody (ab209350)
Immunocytochemical analysis of PPAR gamma using ab209350 at the dilution 1/200. 3T3-L1 cells providing positive signal have been differentiated for 7 days.
PPAR gamma is shown in red, lipid droplets (that indicates the proper differentiation of the cells) are shown in green.
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Western blot - Anti-PPAR gamma antibody (ab209350)All lanes : Anti-PPAR gamma antibody (ab209350)
Lane 1 : NIH/3T3 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : PC-12 cell lysate
Lane 5 : U-87 MG cell lysate
Predicted band size: 57 kDa
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Western blot - Anti-PPAR gamma antibody (ab209350)All lanes : Anti-PPAR gamma antibody (ab209350) at 1/500 dilution
Lane 1 : 3T3
Lane 2 : 3T3-L1 differentiated day 7
Lysates/proteins at 20 µg per lane.
Predicted band size: 57 kDaSpecific band detected in 3T3-L1 day 7 differentiated lysate with no reactivity in 3T3 lysate.
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Immunocytochemistry/ Immunofluorescence - Anti-PPAR gamma antibody (ab209350)Immunocytochemical analysis of PPAR gamma using ab209350 at the dilution 1/200.The image at the top shows 3T3-L1 cells differntiated (for 7 days) where PPAR gamma is shown in green. The image below shows 3T3-L1 undifferentiated cells where no PPAR gamma is detected.
