Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GATA4 antibody [EPR23691-12] - BSA and Azide free (ab275254)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23691-12] to GATA4 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-GATA4 antibody [EPR23691-12] - BSA and Azide free
    See all GATA4 primary antibodies

  • Description

    Rabbit monoclonal [EPR23691-12] to GATA4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2, Huh7 and HEK-293T whole cell lysate. Human liver cancer tissue lysate. IP: Huh7 whole cell lysate.

  • General notes

    ab275254 is the carrier-free version of ab256782. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275254 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GATA4 antibody [EPR23691-12] - BSA and Azide free (ab275254)
    Western blot - Anti-GATA4 antibody [EPR23691-12] - BSA and Azide free (ab275254)
    All lanes : Anti-GATA4 antibody [EPR23691-12] (ab256782) at 1/1000 dilution

    Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 10 µg
    Lane 2 : Huh7 (human hepatocellar carcinoma epithelial cell), whole cell lysate at 20 µg
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), whole cell lysate at 20 µg
    Lane 4 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 44 kDa



    This data was developed using ab256782, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The lane 1 of blot was developed using a higher sensitivity ECL substrate. 

    The molecular weight observed is consistent with what has been described in the literature (PMID: 28758902, 16940177, 18426912, 31903133). 

    20 kDa band in HepG2 whole cell lysate is consistent with what has been described in the literature (PMID: 25501827). 

    Negative control: HeLa (PMID: 24696446, 24352556, 31322241)

    Exposure time: Lane 1: 101 seconds; Lane 2-4: 3 minutes.

  • Immunoprecipitation - Anti-GATA4 antibody [EPR23691-12] - BSA and Azide free (ab275254)
    Immunoprecipitation - Anti-GATA4 antibody [EPR23691-12] - BSA and Azide free (ab275254)

    This data was developed using ab256782, the same antibody clone in a different buffer formulation.

    GATA4 was immunoprecipitated from 0.35 mg Huh7 (human hepatocellular carcinoma epithelial cell), whole cell lysate 10 µg with ab256782 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256782 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Huh7 whole cell lysate 10 µg.

    Lane 2: ab256782 IP in Huh7 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256782 in Huh7 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 49 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 31903133). 20 kDa band in Huh7 whole cell lysate is consistent with what has been described in the literature (PMID: 25501827).

  • Western blot - Anti-GATA4 antibody [EPR23691-12] - BSA and Azide free (ab275254)
    Western blot - Anti-GATA4 antibody [EPR23691-12] - BSA and Azide free (ab275254)
    Anti-GATA4 antibody [EPR23691-12] (ab256782) at 1/1000 dilution + Human liver cancer tissue lysate at 20 µg

    Secondary
    VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 44 kDa



    This data was developed using ab256782, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-GATA4 antibody [EPR23691-12] - BSA and Azide free (ab275254)
    Anti-GATA4 antibody [EPR23691-12] - BSA and Azide free (ab275254)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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