Anti-Bcl6 antibody (ab272859)
Key features and details
- Rabbit polyclonal to Bcl6
- Suitable for: IHC-Fr, WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Bcl6 antibody
See all Bcl6 primary antibodies -
Description
Rabbit polyclonal to Bcl6 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human Bcl6. The exact sequence is proprietary. Center region.
Database link: P41182 -
Positive control
- WB: Neuro2A, GL261, C8D30, NIH-3T3, Raw264.7, Raji and C2C12 whole cell lysate. IHC-P: Human tonsil tissue. IHC-Fr: Mouse cerebellum tissue. IP: Raji whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Anti-Bcl6 antibody (ab272859)
Frozen sections of mouse cerebellum tissue stained for Bcl6 using ab272859 at 1/250 dilution in immunohistochemical analysis. Counterstain: NF-H (red). Fluoroshield™ with DAPI (blue).
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Western blot - Anti-Bcl6 antibody (ab272859)All lanes : Anti-Bcl6 antibody (ab272859) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : GL261 (mouse glioblastoma cancer cell line) whole cell lysate
Lane 3 : C8-D30 (mouse astrocyte type III cerebellum cell line) whole cell lysate
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 5 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 6 : C2C12 (mouse myoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody7.5% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody (ab272859)Paraffin embedded human tonsil tissue stained for Bcl6 using ab272859 at 10 μg/ml in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
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Western blot - Anti-Bcl6 antibody (ab272859)All lanes : Anti-Bcl6 antibody (ab272859) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody7.5% SDS-PAGE
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Immunoprecipitation - Anti-Bcl6 antibody (ab272859)7.5% SDS-PAGE.
Immunoprecipitation of BCL6 from Raji (human Burkitt's lymphoma cell line) whole cell lysate by ab272859, 3 ug.
All lanes : Anti-Bcl6 antibody (ab272859) at 1/1000 dilution
Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Lane 2 : Control: pre-immune rabbit IgG at 3 µg
Lane 3 : Immunoprecipitation of BCL6 by ab272859 at 3 µg
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
