8-iso-PGF2alpha ELISA Kit (ab133043)
Key features and details
- Sensitivity: 40 pg/ml
- Range: 160 pg/ml - 100000 pg/ml
- Sample type: Plasma, Serum, Tissue
- Detection method: Colorimetric
- Assay type: Competitive
Overview
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Product name
8-iso-PGF2alpha ELISA Kit
See all 8-iso-PGF2a kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 970 pg/mL 16 11.3% 3264 pg/mL 16 5.7% 5092 pg/mL 16 5.1% Inter-assay Sample n Mean SD CV% 1473 pg/mL 8 5.4% 4501 pg/mL 8 5.8% 6679 pg/mL 8 10.4% -
Sample type
Serum, Plasma, Tissue -
Assay type
Competitive -
Sensitivity
40 pg/ml -
Range
160 pg/ml - 100000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 93.8 % - % Hep Plasma 97.2 % - % EDTA Plasma 109.8 % - % -
Assay time
3h 00m -
Assay duration
Multiple steps standard assay -
Product overview
Abcam’s Direct 8-iso-PGF2alpha in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 8-iso-PGF2α in Biological fluids.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-8-iso-PGF2alpha antigen and a rabbit polyclonal antibody specific to 8-iso-PGF2alpha. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 8-iso-PGF2alpha captured in the plate.
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Notes
The 8-epimer of Prostaglandin F2α (8-iso-PGF2α) is produced in vivo by both non-cyclooxygenase and cyclooxygenase dependent mechanisms from arachidonic acid. 8-iso-PGF2α has been shown to be a potent vasoconstrictor, a potential mediator of hepatorenal syndrome and atherosclerosis and a mutagen in 3T3 cells and in vascular smooth muscle cells. It has also been postulated to participate as a pathophysiological mediator and is able to modify the fluidity and integrity of membranes. 8-isoPGF2α has been shown to circulate in plasma and is excreted in urine. Methods for assessing total 8-iso-PGF2α typically require the alkaline hydrolysis of 8-iso-PGF2α esters from tissues, followed by length procedures involving extractions, phase separations and thin layer chromatography.
Cross Reactivity
Compound % Cross Reactivity 8-iso-PGF2α 100 PGF1α 4.6 PGF2α 1.85 PGE1 0.19 TXB2 0.023 PGB1 0.02 PGE3 0.012 6-keto-PGF1α 0.008 13,14-dihydro-15-keto-PGF2α 0.008 6,15-keto-13,14-dihydro-PGF1α 0.005 8-iso-PGE1 PGA2 PGJ2 2-Arachidonoylglycerol Anandamide -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml 8-iso-PGF2 alpha Standard 1 x 0.5ml Direct 8-iso-PGF2 alpha Alkaline Phosphatase Conjugate 1 x 5ml Direct 8-iso-PGF2 alpha Antibody 1 x 5ml Direct 8-iso-PGF2 alpha Sample Diluent 1 x 27ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Neutralizing Reagent 1 x 5ml Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml -
Research areas
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Alternative names
- 8-iso-Prostaglandin F2a
Images
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Typical Standard Curve - 2 Hour Format
Representative Standard Curve using ab133043. 2 Hour Format.
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Typical Standard Curve - Overnight FormatRepresentative Standard Curve using ab133043. Overnight Format.
