8-iso-PGF2 alpha ELISA Kit (ab133025)
Key features and details
- Sensitivity: 16.3 pg/ml
- Range: 6.1 pg/ml - 100000 pg/ml
- Sample type: Cell culture supernatant, Tissue, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
8-iso-PGF2 alpha ELISA Kit
See all 8-iso-PGF2a kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Low 12 232pg/ml 11% Medium 12 1128pg/ml 4.4% High 12 8263pg/ml 5.8% Inter-assay Sample n Mean SD CV% Low 8 183pg/ml 11% Medium 8 855pg/ml 8.8% High 8 6239pg/ml 5% -
Sample type
Cell culture supernatant, Urine, Tissue -
Assay type
Competitive -
Sensitivity
16.3 pg/ml -
Range
6.1 pg/ml - 100000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 101.58 % - % Tissue Culture Media 129.9 % - % -
Assay time
3h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Predicted to work with: a wide range of other species
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Product overview
Abcam’s 8-iso-PGF2 alpha in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 8-iso-PGF2 alpha in tissue culture media and urine.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-8-iso-PGF2 alpha antigen and a polyclonal rabbit antibody specific to 8-iso-PGF2 alpha. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 8-iso-PGF2 alpha captured in the plate.
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Notes
The measurement of F2-isoprostanes has emerged as one of the most reliable approaches to assess oxidative stress status in vivo, providing an important tool to explore the role of oxidative stress in the pathogenesis of human disease. This is a colorimetric competitive immunoassay kit for the quantitative determination of free 8-iso-Prostaglandin F2α in biological fluids such as urine and in culture supernatants. 8-iso-PGF2α is produced in vivo by both non-cyclooxygenase and cyclooxygenase dependant mechanisms from arachidonic acid. It has been shown to be a potent vasoconstrictor, a potential mediator of hepatorenal syndrome and a mutagen in 3T3 cells and vascular smooth muscle cells. 8-iso-PGF2α has been shown to circulate in plasma and is excreted in urine.
Compound Cross Reactivity 8-iso-PGF2α 100% PGF1α 4.6% PGF2α 1.85% PGE1 0.19% TXB2 0.023% PGB1 0.02% PGE3 0.012% 6-keto-PGF1α 0.008% 13, 14-dihydro-15-keto-PGF2α 0.005% 6,15-keto-13, 14-dihydro-PGF1α 8-iso-PGE1 PGA2 PGJ2 2-Arachidonoylglycerol Anandamide -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Assay Buffer 1 x 27ml 20X Wash Buffer Concentrate 1 x 27ml 8-iso-PGF2 alpha Alkaline Phosphatase Conjugate 1 x 5ml 8-iso-PGF2 alpha EIA Antibody 1 x 5ml 8-iso-PGF2 alpha Standard 1 x 500µl Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml -
Research areas
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Alternative names
- 8-iso-Prostaglandin F2a
Images
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Typical Standard Curve
Representative Standard Curve using ab133025
