Anti-Gelsolin antibody (ab214342)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alpaca monoclonal to Gelsolin
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-Gelsolin antibody
See all Gelsolin primary antibodies -
Description
Alpaca monoclonal to Gelsolin -
Host species
Alpaca -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human Gelsolin.
Database link: P06396 -
General notes
Contains a FLAG tag (DDDDK) and a His tag Therefore, an anti DDDDK or anti His secondary can be used. We recommend the anti-FLAG option (ab49763).
Please note: ab214342 only recognises the Ca2+ activated form of Human Gelsolin.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: PBS, 2.9% Sodium chloride -
Concentration information loading... -
Purity
Purified via His tag -
Clonality
Monoclonal -
Research areas
Images
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Immunoprecipitation - Anti-Gelsolin antibody (ab214342)
Anti-Gelsolin antibody (ab214342) was conjugated to NHS activated magnetic beads at 1 mg of antibody per 1 ml of slurry.
20 ul of resulting slurry was combined with 200 ug of protein extracted from HeLa cells using RIPA buffer.
Samples were analysed by western blot using anti Gelsolin primary antibody (ab74420 at 1 ug/ml) and anti Rabbit secondary antibody (ab201489 at 1/50000).
Lane 1: 10% input
Lane 2: Immunoprecipitated material
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Western blot - Anti-Gelsolin antibody (ab214342)All lanes : Anti-Gelsolin antibody (ab214342) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Human kidney tissue lysate - total protein
Lane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence) (ab49763) at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 86 kDa
Observed band size: 86 kDa
Exposure time: 10 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with abX overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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Anti-Gelsolin antibody (ab214342)
