Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DAG1 antibody [IIH6C4] (ab234587)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [IIH6C4] to DAG1
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DAG1 antibody [IIH6C4]
    See all DAG1 primary antibodies

  • Description

    Mouse monoclonal [IIH6C4] to DAG1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Human, Rat, Mouse skeletal muscle, Human heart. IHC-P: Human pancreas, skeletal muscle, stomach, Mouse kidney, Rat stomach. IHC-Fr: Mouse and Rat skeletal muscle.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-DAG1 antibody [IIH6C4] (ab234587)
    Western blot - Anti-DAG1 antibody [IIH6C4] (ab234587)
    All lanes : Anti-DAG1 antibody [IIH6C4] (ab234587) at 1/5000 dilution

    Lane 1 : Human skeletal muscle
    Lane 2 : Human heart
    Lane 3 : Rat skeletal muscle
    Lane 4 : Mouse skeletal muscle

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 97 kDa
    Observed band size: 120/150 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST.

    Lanes 1-3: 20 seconds Lane 4: 3 minutes.

    Lanes 1-3 used the 6% gel and lane 4 used the 4-20% gradient gel.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 31566294 )

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] (ab234587)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] (ab234587)

    Immunohistochemical analysis of paraffin-embedded sections human pancreas tissue labeling DAG1 with ab234587 at 1/500 dilution, followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Membrane staining on human pancreas is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] (ab234587)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] (ab234587)

    Immunohistochemical analysis of paraffin-embedded sections human skeletal muscle tissue labeling DAG1 with ab234587 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membrane staining on human skeletal muscle is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] (ab234587)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] (ab234587)

    Immunohistochemical analysis of paraffin-embedded sections human stomach tissue labeling DAG1 with ab234587 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membrane staining on human stomach is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] (ab234587)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] (ab234587)

    Immunohistochemical analysis of paraffin-embedded sections mouse kidney tissue labeling DAG1 with ab234587 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on mouse kidney is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] (ab234587)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] (ab234587)

    Immunohistochemical analysis of paraffin-embedded sections rat stomach tissue labeling DAG1 with ab234587 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membrane staining on rat stomach is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

  • Immunohistochemistry (Frozen sections) - Anti-DAG1 antibody [IIH6C4] (ab234587)
    Immunohistochemistry (Frozen sections) - Anti-DAG1 antibody [IIH6C4] (ab234587)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized mouse skeletal muscle frozen sections labeling DAG1 with ab234587 at 1/100 dilution, followed by Goat Anti-Mouse (Alexa Fluor® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on mouse skeletal muscle. The nuclear counter stain is DAPI (blue).

     

  • Immunohistochemistry (Frozen sections) - Anti-DAG1 antibody [IIH6C4] (ab234587)
    Immunohistochemistry (Frozen sections) - Anti-DAG1 antibody [IIH6C4] (ab234587)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat skeletal muscle frozen sections labeling DAG1 with ab234587 at 1/100 dilution, followed by Goat Anti-Mouse (Alexa Fluor® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on rat skeletal muscle. The nuclear counter stain is DAPI (blue).

  • Anti-DAG1 antibody [IIH6C4] (ab234587)
    Anti-DAG1 antibody [IIH6C4] (ab234587)

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