Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µg 321 638 ₸ 10 µg 110 563 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [IIH6C4] to DAG1
Suitable for: WB, IHC-P, IHC-Fr
Reacts with: Mouse, Rat, Human

Product name

Anti-DAG1 antibody [IIH6C4]
See all DAG1 primary antibodies
Description

Mouse monoclonal [IIH6C4] to DAG1
Host species

Mouse

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant full length protein. This information is considered to be commercially sensitive.
Positive control
- WB: Human, Rat, Mouse skeletal muscle, Human heart. IHC-P: Human pancreas, skeletal muscle, stomach, Mouse kidney, Rat stomach. IHC-Fr: Mouse and Rat skeletal muscle.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

IIH6C4
Isotype

IgM
Research areas

Western blot - Anti-DAG1 antibody [IIH6C4] (ab234587)

All lanes : Anti-DAG1 antibody [IIH6C4] (ab234587) at 1/5000 dilution

Lane 1 : Human skeletal muscle
Lane 2 : Human heart
Lane 3 : Rat skeletal muscle
Lane 4 : Mouse skeletal muscle

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 97 kDa
Observed band size: 120/150 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST.

Lanes 1-3: 20 seconds Lane 4: 3 minutes.

Lanes 1-3 used the 6% gel and lane 4 used the 4-20% gradient gel.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 31566294 )
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] (ab234587)

Immunohistochemical analysis of paraffin-embedded sections human pancreas tissue labeling DAG1 with ab234587 at 1/500 dilution, followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Membrane staining on human pancreas is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] (ab234587)

Immunohistochemical analysis of paraffin-embedded sections human skeletal muscle tissue labeling DAG1 with ab234587 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membrane staining on human skeletal muscle is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] (ab234587)

Immunohistochemical analysis of paraffin-embedded sections human stomach tissue labeling DAG1 with ab234587 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membrane staining on human stomach is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] (ab234587)

Immunohistochemical analysis of paraffin-embedded sections mouse kidney tissue labeling DAG1 with ab234587 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on mouse kidney is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] (ab234587)

Immunohistochemical analysis of paraffin-embedded sections rat stomach tissue labeling DAG1 with ab234587 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membrane staining on rat stomach is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.
Immunohistochemistry (Frozen sections) - Anti-DAG1 antibody [IIH6C4] (ab234587)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized mouse skeletal muscle frozen sections labeling DAG1 with ab234587 at 1/100 dilution, followed by Goat Anti-Mouse (Alexa Fluor^® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on mouse skeletal muscle. The nuclear counter stain is DAPI (blue).
Immunohistochemistry (Frozen sections) - Anti-DAG1 antibody [IIH6C4] (ab234587)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat skeletal muscle frozen sections labeling DAG1 with ab234587 at 1/100 dilution, followed by Goat Anti-Mouse (Alexa Fluor^® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on rat skeletal muscle. The nuclear counter stain is DAPI (blue).
Anti-DAG1 antibody [IIH6C4] (ab234587)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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