Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DAG1 antibody [IIH6C4] - BSA and Azide free (ab255738)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [IIH6C4] to DAG1 - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DAG1 antibody [IIH6C4] - BSA and Azide free
    See all DAG1 primary antibodies

  • Description

    Mouse monoclonal [IIH6C4] to DAG1 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • IHC-P: Human pancreas, skeletal muscle, stomach, Mouse kidney, Rat stomach. IHC-Fr: Mouse and Rat skeletal muscle.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255738 is the carrier-free version of ab234587. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] - BSA and Azide free (ab255738)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] - BSA and Azide free (ab255738)

    Immunohistochemical analysis of paraffin-embedded sections human pancreas tissue labeling DAG1 with ab234587 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membrane staining on human pancreas is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab234587).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] - BSA and Azide free (ab255738)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] - BSA and Azide free (ab255738)

    Immunohistochemical analysis of paraffin-embedded sections human skeletal muscle tissue labeling DAG1 with ab234587 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membrane staining on human skeletal muscle is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab234587).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] - BSA and Azide free (ab255738)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] - BSA and Azide free (ab255738)

    Immunohistochemical analysis of paraffin-embedded sections human stomach tissue labeling DAG1 with ab234587 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membrane staining on human stomach is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab234587).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] - BSA and Azide free (ab255738)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] - BSA and Azide free (ab255738)

    Immunohistochemical analysis of paraffin-embedded sections mouse kidney tissue labeling DAG1 with ab234587 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on mouse kidney is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab234587).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] - BSA and Azide free (ab255738)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] - BSA and Azide free (ab255738)

    Immunohistochemical analysis of paraffin-embedded sections rat stomach tissue labeling DAG1 with ab234587 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membrane staining on rat stomach is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab234587).

  • Immunohistochemistry (Frozen sections) - Anti-DAG1 antibody [IIH6C4] - BSA and Azide free (ab255738)
    Immunohistochemistry (Frozen sections) - Anti-DAG1 antibody [IIH6C4] - BSA and Azide free (ab255738)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized mouse skeletal muscle frozen sections labeling DAG1 with ab234587 at 1/100 dilution, followed by Goat Anti-Mouse (Alexa Fluor® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on mouse skeletal muscle. The nuclear counter stain is DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab234587).

  • Immunohistochemistry (Frozen sections) - Anti-DAG1 antibody [IIH6C4] - BSA and Azide free (ab255738)
    Immunohistochemistry (Frozen sections) - Anti-DAG1 antibody [IIH6C4] - BSA and Azide free (ab255738)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat skeletal muscle frozen sections labeling DAG1 with ab234587 at 1/100 dilution, followed by Goat Anti-Mouse (Alexa Fluor® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on rat skeletal muscle. The nuclear counter stain is DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab234587).

  • Anti-DAG1 antibody [IIH6C4] - BSA and Azide free (ab255738)
    Anti-DAG1 antibody [IIH6C4] - BSA and Azide free (ab255738)

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