Human Ezrin ELISA Kit (ab278116)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 19.37 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Ezrin ELISA Kit
See all Ezrin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.5% Inter-assay Sample n Mean SD CV% Serum 3 7.6% -
Sample type
Serum, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
19.37 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 94 84% - 104% Hep Plasma 85 80% - 91% EDTA Plasma 91 80% - 100% Cit plasma 84 80% - 87% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Ezrin ELISA kit (ab2778116) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Ezrin protein in human serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Ezrin with 19.37 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Ezrin is a cytoplasmic peripheral protein involved in adhesion, migration, and organization of cytoskeletal structures to the plasma membrane. In epithelial cells, Ezrin is required for the formation of microvilli and membrane ruffles on the apical pole. The N-terminal domain of Ezrin binds to NHERF protein involved in long-range allostery. Along with PLEKHG6, Ezrin is required for normal macropinocytosis. Human Ezrin holds a 97% and 96% sequence homology with mouse and rat Ezrin, respectively.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Ezrin Capture Antibody 1 x 600µl 10X Human Ezrin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Ezrin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. -
Tissue specificity
Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied. -
Sequence similarities
Contains 1 FERM domain. -
Developmental stage
Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain. -
Post-translational
modificationsPhosphorylated by tyrosine-protein kinases. -
Cellular localization
Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein. - Information by UniProt
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Alternative names
- Villin 2 ezrin
- CVIL
- CVL
see all -
Database links
- Entrez Gene: 7430 Human
- Omim: 123900 Human
- SwissProt: P15311 Human
- Unigene: 487027 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Recombinant Antibody benefitsTo learn more about the advantages of recombinant antibodies see here.
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Example of human Ezrin standard curve in Sample Diluent NS. -
Interpolated concentrations of native Ezrin in human serum and plasma samples.The concentrations of Ezrin were measured in duplicates, interpolated from the Ezrin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 50%, plasma (heparin) 100% and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ezrin concentration was determined to be 3409.74 pg/mL in serum, 1833.59 pg/mL in plasma (citrate), 3172.31 pg/mL in plasma (EDTA), and 3470.33 pg/mL in plasma (heparin).
