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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Binding Proteins

Anti-Gelsolin antibody [EP1940Y] (ab134183)

Price and availability

288 134 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-Gelsolin antibody [EP1940Y] (ab134183)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit monoclonal [EP1940Y] to Gelsolin
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Gelsolin antibody [EP1940Y]
    See all Gelsolin primary antibodies
  • Description

    Rabbit monoclonal [EP1940Y] to Gelsolin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human Gelsolin aa 650-750 (C terminal).
    Database link: P06396

  • Positive control

    • NIH3T3 cell lysate; Human tonsil tissue
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP1940Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins

Images

  • Western blot - Anti-Gelsolin antibody [EP1940Y] (ab134183)
    Western blot - Anti-Gelsolin antibody [EP1940Y] (ab134183)
    All lanes : Anti-Gelsolin antibody [EP1940Y] (ab134183) at 1/10000 dilution

    Lane 1 : THP-1 whole cell lysate
    Lane 2 : THP-1 cultured medium
    Lane 3 : MCF-7 whole cell lysate
    Lane 4 : Human plasma

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 86 kDa
    Observed band size: 81/86 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blockiing buffer 5% NFDM/TBST
    Diluting buffer 5% NFDM/TBST

    The secretory isoform of gelsolin is at 86kDa. The cytoplasmic isoforms of gelsolin are at 81kDa.

  • Western blot - Anti-Gelsolin antibody [EP1940Y] (ab134183)
    Western blot - Anti-Gelsolin antibody [EP1940Y] (ab134183)
    Anti-Gelsolin antibody [EP1940Y] (ab134183) at 1/5000 dilution + NIH3T3 cell lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 86 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody [EP1940Y] (ab134183)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody [EP1940Y] (ab134183)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling Gelsolin with ab134183 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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