Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸ 10 µl 123 964 ₸

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Anti-Gelsolin antibody [EPR1942] (ab109014)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1942] to Gelsolin
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-Gelsolin antibody [EPR1942]
See all Gelsolin primary antibodies
Description

Rabbit monoclonal [EPR1942] to Gelsolin
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- MCF-7, HL-60, A431, and HeLa cell lysates; Human kidney tissue; Human lung tissue; HeLa cells
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR1942
Isotype

IgG
Research areas

Western blot - Anti-Gelsolin antibody [EPR1942] (ab109014)

All lanes : Anti-Gelsolin antibody [EPR1942] (ab109014) at 1/20000 dilution

Lane 1 : THP-1 whole cell lysate
Lane 2 : THP-1 cultured medium
Lane 3 : MCF-7 whole cell lysate
Lane 4 : Human Plasma

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 86 kDa
Observed band size: 81/86 kDa
why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking buffer 5% NFDM/TBST
Diluting buffer 5% NFDM/TBST

The secretory isoform of gelsolin is at 86kDa. The cytoplasmic isoforms of gelsolin are at 81kDa.
Western blot - Anti-Gelsolin antibody [EPR1942] (ab109014)

All lanes : Anti-Gelsolin antibody [EPR1942] (ab109014) at 1/50000 dilution

Lane 1 : MCF7 cell lysate
Lane 2 : HL60 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 86 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody [EPR1942] (ab109014)

Immunohistochemical analysis of Gelsolin expression in paraffin-embedded human kidney tissue using ab109014 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody [EPR1942] (ab109014)

Immunohistochemical analysis of Gelsolin expression in paraffin-embedded Human lung tissue using ab109014 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Gelsolin antibody [EPR1942] (ab109014)

Immunofluorescent staining of Gelsolin in HeLa cells using ab109014 at 1/100 dilution.
Flow Cytometry - Anti-Gelsolin antibody [EPR1942] (ab109014)

Overlay histogram showing HL60 cells stained with ab109014 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109014, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Anti-Gelsolin antibody [EPR1942] (ab109014)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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