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Anti-Gelsolin antibody [GS-2C4] (ab11081)

Key features and details

  • Mouse monoclonal [GS-2C4] to Gelsolin
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Gelsolin antibody [GS-2C4]
    See all Gelsolin primary antibodies

  • Description

    Mouse monoclonal [GS-2C4] to Gelsolin

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein (purified) corresponding to Human Gelsolin.
    Database link: P06396-1

  • Epitope

    The antibody is directed against an epitope located on the 47 kD peptide derived from a chymotryptic cleavage of gelsolin. This peptide contains the carboxy terminal actin binding site for gelsolin.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Images

  • Western blot - Anti-Gelsolin antibody [GS-2C4] (ab11081)
    Western blot - Anti-Gelsolin antibody [GS-2C4] (ab11081)
    Anti-Gelsolin antibody [GS-2C4] (ab11081) at 1/2000 dilution + Human Plasma

    Secondary
    AP conjugated anti-mouse Fab IgG

    Performed under reducing conditions.

    Predicted band size: 86 kDa



    This image was generated using the ascites version of the product.

  • Immunocytochemistry/ Immunofluorescence - Anti-Gelsolin antibody [GS-2C4] (ab11081)
    Immunocytochemistry/ Immunofluorescence - Anti-Gelsolin antibody [GS-2C4] (ab11081)

    ICC/IF image of ab11081 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11081, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.

    This image was generated using the ascites version of the product.

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