Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µg 307 566 ₸ 10 µg 186 952 ₸

Order now and get it on Tuesday March 16, 2021

Anti-Alpha Dystroglycan antibody [45-3] (ab199768)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [45-3] to Alpha Dystroglycan
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat

Product name

Anti-Alpha Dystroglycan antibody [45-3]
See all Alpha Dystroglycan primary antibodies
Description

Rabbit monoclonal [45-3] to Alpha Dystroglycan
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Mouse

Rat

WB
Mouse

Rat

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Rat and mouse placenta, heart, and brain tissue lysates. Mouse skeletal muscle tissue lysate. Neuro-2a cell lysate. IHC-P: Rat placenta tissue, Mouse skeletal muscle tissue.
General notes
This product has switched from a hybridoma to recombinant production method on 9th September 2020.

This antibody clone is manufactured by Abcam.

If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
IHC-P	Mouse Rat
WB	Mouse Rat

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

45-3
Isotype

IgG
Research areas

Western blot - Anti-Alpha Dystroglycan antibody [45-3] (ab199768)

All lanes : Anti-Alpha Dystroglycan antibody [45-3] (ab199768) at 1 µg/ml

Lane 1 : Human Brain tissue lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Mouse heart tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat heart tissue lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 97 kDa
Observed band size: 75-100 kDa
why is the actual band size different from the predicted?

Lanes 1 - 6: Merged signal (red and green). Green - ab199768 observed at 75-100 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab199768 was shown to react with DAG1 in Western blot. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab199768 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Dystroglycan antibody [45-3] (ab199768)

Immunohistochemical analysis of paraffin-embedded Rat placenta tissues labeling Alpha Dystroglycan with ab199768 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).
Western blot - Anti-Alpha Dystroglycan antibody [45-3] (ab199768)

All lanes : Anti-Alpha Dystroglycan antibody [45-3] (ab199768) at 1000 µg/ml

Lane 1 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
Lane 2 : Mouse placenta lysate
Lane 3 : Rat placenta lysate
Lane 4 : Rat heart lysate
Lane 5 : Rat brain lysate
Lane 6 : Mouse skeletal muscle lysate
Lane 7 : Mouse heart lysate
Lane 8 : Mouse brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 97 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?

Exposure time: 125 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Dystroglycan antibody [45-3] (ab199768)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissues labeling Alpha Dystroglycan with ab199768 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).
Anti-Alpha Dystroglycan antibody [45-3] (ab199768)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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