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Anti-Alpha Dystroglycan antibody [45-3] (ab199768)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [45-3] to Alpha Dystroglycan
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Alpha Dystroglycan antibody [45-3]
    See all Alpha Dystroglycan primary antibodies

  • Description

    Rabbit monoclonal [45-3] to Alpha Dystroglycan

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat and mouse placenta, heart, and brain tissue lysates. Mouse skeletal muscle tissue lysate. Neuro-2a cell lysate. IHC-P: Rat placenta tissue, Mouse skeletal muscle tissue.

  • General notes

    This product has switched from a hybridoma to recombinant production method on 9th September 2020.

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Alpha Dystroglycan antibody [45-3] (ab199768)
    Western blot - Anti-Alpha Dystroglycan antibody [45-3] (ab199768)
    All lanes : Anti-Alpha Dystroglycan antibody [45-3] (ab199768) at 1 µg/ml

    Lane 1 : Human Brain tissue lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Mouse heart tissue lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Rat heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 97 kDa
    Observed band size: 75-100 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 6: Merged signal (red and green). Green - ab199768 observed at 75-100 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab199768 was shown to react with DAG1 in Western blot. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab199768 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Dystroglycan antibody [45-3] (ab199768)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Dystroglycan antibody [45-3] (ab199768)

    Immunohistochemical analysis of paraffin-embedded Rat placenta tissues labeling Alpha Dystroglycan with ab199768 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).

  • Western blot - Anti-Alpha Dystroglycan antibody [45-3] (ab199768)
    Western blot - Anti-Alpha Dystroglycan antibody [45-3] (ab199768)
    All lanes : Anti-Alpha Dystroglycan antibody [45-3] (ab199768) at 1000 µg/ml

    Lane 1 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
    Lane 2 : Mouse placenta lysate
    Lane 3 : Rat placenta lysate
    Lane 4 : Rat heart lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Mouse skeletal muscle lysate
    Lane 7 : Mouse heart lysate
    Lane 8 : Mouse brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 97 kDa
    Observed band size: 95 kDa why is the actual band size different from the predicted?


    Exposure time: 125 seconds


    Blocking and diluting buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Dystroglycan antibody [45-3] (ab199768)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Dystroglycan antibody [45-3] (ab199768)

    Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissues labeling Alpha Dystroglycan with ab199768 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).

  • Anti-Alpha Dystroglycan antibody [45-3] (ab199768)
    Anti-Alpha Dystroglycan antibody [45-3] (ab199768)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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