Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Alpha Dystroglycan antibody [45-3] - BSA and Azide free (ab242442)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [45-3] to Alpha Dystroglycan - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Alpha Dystroglycan antibody [45-3] - BSA and Azide free
    See all Alpha Dystroglycan primary antibodies

  • Description

    Rabbit monoclonal [45-3] to Alpha Dystroglycan - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse placenta, heart, skeletal muscle and brain lysates; rat placenta, heart and brain lysates; Neuro-2a whole cell lysate; Human brain tissue lysate. IHC-P: Mouse skeletal muscle tissue; rat placenta tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Alpha Dystroglycan antibody [45-3] - BSA and Azide free (ab242442)
    Western blot - Anti-Alpha Dystroglycan antibody [45-3] - BSA and Azide free (ab242442)
    All lanes : Anti-Alpha Dystroglycan antibody [45-3] (ab199768) at 1 µg/ml

    Lane 1 : Human Brain tissue lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Mouse heart tissue lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Rat heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 97 kDa
    Observed band size: 75-100 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab199768).

    Lanes 1 - 6: Merged signal (red and green). Green - ab199768 observed at 75-100 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab199768 was shown to react with DAG1 in Western blot. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab199768 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-Alpha Dystroglycan antibody [45-3] - BSA and Azide free (ab242442)
    Western blot - Anti-Alpha Dystroglycan antibody [45-3] - BSA and Azide free (ab242442)
    All lanes : Anti-Alpha Dystroglycan antibody [45-3] - BSA and Azide free (ab242442) at 1/1000 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
    Lane 2 : Mouse placenta lysate
    Lane 3 : Rat placenta lysate
    Lane 4 : Rat heart lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Mouse skeletal muscle lysate
    Lane 7 : Mouse heart lysate
    Lane 8 : Mouse brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 97 kDa
    Observed band size: 95-150 kDa why is the actual band size different from the predicted?


    Exposure time: 125 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

    The predicted band size is 97 kDa, the molecular weight observed is because of glycosylation.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Dystroglycan antibody [45-3] - BSA and Azide free (ab242442)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Dystroglycan antibody [45-3] - BSA and Azide free (ab242442)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Alpha Dystroglycan with ab252442 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Extracellular matrix staining on mouse skeletal muscle (PMID: 29855340, PMID: 18691338) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Dystroglycan antibody [45-3] - BSA and Azide free (ab242442)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Dystroglycan antibody [45-3] - BSA and Azide free (ab242442)

    Immunohistochemical analysis of paraffin-embedded rat placenta tissue labeling Alpha Dystroglycan with ab252442 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Extracellular matrix staining on endothelium of rat placenta (PMID: 29855340, PMID: 18691338) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-Alpha Dystroglycan antibody [45-3] - BSA and Azide free (ab242442)
    Anti-Alpha Dystroglycan antibody [45-3] - BSA and Azide free (ab242442)

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