Antibodies, Proteins, Kits and Reagents for Life Science

526 012 ₸ Product size

100 µg 526 012 ₸ 1 mg 4 690 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Alpha Dystroglycan antibody [45-3] - BSA and Azide free (ab242442)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [45-3] to Alpha Dystroglycan - BSA and Azide free
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Alpha Dystroglycan antibody [45-3] - BSA and Azide free
See all Alpha Dystroglycan primary antibodies
Description

Rabbit monoclonal [45-3] to Alpha Dystroglycan - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse placenta, heart, skeletal muscle and brain lysates; rat placenta, heart and brain lysates; Neuro-2a whole cell lysate; Human brain tissue lysate. IHC-P: Mouse skeletal muscle tissue; rat placenta tissue.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF^®).

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

45-3
Isotype

IgG
Research areas

Western blot - Anti-Alpha Dystroglycan antibody [45-3] - BSA and Azide free (ab242442)

All lanes : Anti-Alpha Dystroglycan antibody [45-3] (ab199768) at 1 µg/ml

Lane 1 : Human Brain tissue lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Mouse heart tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat heart tissue lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 97 kDa
Observed band size: 75-100 kDa
why is the actual band size different from the predicted?

This data was developed using the same antibody clone in a different buffer formulation (ab199768).

Lanes 1 - 6: Merged signal (red and green). Green - ab199768 observed at 75-100 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab199768 was shown to react with DAG1 in Western blot. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab199768 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-Alpha Dystroglycan antibody [45-3] - BSA and Azide free (ab242442)

All lanes : Anti-Alpha Dystroglycan antibody [45-3] - BSA and Azide free (ab242442) at 1/1000 dilution

Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : Mouse placenta lysate
Lane 3 : Rat placenta lysate
Lane 4 : Rat heart lysate
Lane 5 : Rat brain lysate
Lane 6 : Mouse skeletal muscle lysate
Lane 7 : Mouse heart lysate
Lane 8 : Mouse brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 97 kDa
Observed band size: 95-150 kDa why is the actual band size different from the predicted?

Exposure time: 125 seconds

Blocking and dilution buffer: 5% NFDM/TBST.

The predicted band size is 97 kDa, the molecular weight observed is because of glycosylation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Dystroglycan antibody [45-3] - BSA and Azide free (ab242442)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Alpha Dystroglycan with ab252442 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Extracellular matrix staining on mouse skeletal muscle (PMID: 29855340, PMID: 18691338) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Dystroglycan antibody [45-3] - BSA and Azide free (ab242442)

Immunohistochemical analysis of paraffin-embedded rat placenta tissue labeling Alpha Dystroglycan with ab252442 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Extracellular matrix staining on endothelium of rat placenta (PMID: 29855340, PMID: 18691338) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-Alpha Dystroglycan antibody [45-3] - BSA and Azide free (ab242442)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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