Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ACTR1B antibody [EPR16969] - BSA and Azide free (ab251406)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16969] to ACTR1B - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC, WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-ACTR1B antibody [EPR16969] - BSA and Azide free
    See all ACTR1B primary antibodies

  • Description

    Rabbit monoclonal [EPR16969] to ACTR1B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC, WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251406 is the carrier-free version of ab203835.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ACTR1B antibody [EPR16969] - BSA and Azide free (ab251406)
    Western blot - Anti-ACTR1B antibody [EPR16969] - BSA and Azide free (ab251406)
    All lanes : Anti-ACTR1B antibody [EPR16969] (ab203835) at 1/10000 dilution

    Lane 1 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate
    Lane 4 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 10 seconds


    This data was developed using ab203835, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ACTR1B antibody [EPR16969] - BSA and Azide free (ab251406)
    Western blot - Anti-ACTR1B antibody [EPR16969] - BSA and Azide free (ab251406)
    All lanes : Anti-ACTR1B antibody [EPR16969] (ab203835) at 1/10000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 15 seconds


    This data was developed using ab203835, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ACTR1B antibody [EPR16969] - BSA and Azide free (ab251406)
    Western blot - Anti-ACTR1B antibody [EPR16969] - BSA and Azide free (ab251406)
    Anti-ACTR1B antibody [EPR16969] (ab203835) at 1/1000 dilution + Full length Human ACTR1A recombination protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 42 kDa


    Exposure time: 3 minutes


    This data was developed using ab203835, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTR1B antibody [EPR16969] - BSA and Azide free (ab251406)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTR1B antibody [EPR16969] - BSA and Azide free (ab251406)
    This data was developed using ab203835, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling ACTR1B with ab203835 at 1/300 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-ACTR1B antibody [EPR16969] - BSA and Azide free (ab251406)
    Immunocytochemistry - Anti-ACTR1B antibody [EPR16969] - BSA and Azide free (ab251406)
    This data was developed using ab203835, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling ACTR1B with ab203835 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).The negative controls are as follows:--ve control 1: ab203835 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
  • Immunocytochemistry - Anti-ACTR1B antibody [EPR16969] - BSA and Azide free (ab251406)
    Immunocytochemistry - Anti-ACTR1B antibody [EPR16969] - BSA and Azide free (ab251406)
    This data was developed using ab203835, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling ACTR1B with ab203835 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).The negative controls are as follows:--ve control 1: ab203835 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
  • Flow Cytometry - Anti-ACTR1B antibody [EPR16969] - BSA and Azide free (ab251406)
    Flow Cytometry - Anti-ACTR1B antibody [EPR16969] - BSA and Azide free (ab251406)
    This data was developed using ab203835, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling ACTR1B with ab203835 at 1/300 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
  • Immunoprecipitation - Anti-ACTR1B antibody [EPR16969] - BSA and Azide free (ab251406)
    Immunoprecipitation - Anti-ACTR1B antibody [EPR16969] - BSA and Azide free (ab251406)
    This data was developed using ab203835, the same antibody clone in a different buffer formulation.ACTR1B was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab203835 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab203835 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution. Lane 1: HeLa whole cell lysate 10ug (Input). Lane 2: ab203835 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203835 in HeLa whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 5 seconds.
  • Anti-ACTR1B antibody [EPR16969] - BSA and Azide free (ab251406)
    Anti-ACTR1B antibody [EPR16969] - BSA and Azide free (ab251406)

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