Anti-Ezrin (phospho Y478) antibody (ab79256)
Key features and details
- Rabbit polyclonal to Ezrin (phospho Y478)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ezrin (phospho Y478) antibody
See all Ezrin primary antibodies -
Description
Rabbit polyclonal to Ezrin (phospho Y478) -
Host species
Rabbit -
Specificity
Binds to Ezrin only when phosphorylated at tyrosine 478. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phosphopeptide derived from Human Ezrin around the phosphorylation site of tyrosine 478 (P-V-YP-E-P)
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Positive control
- Extracts form HeLa cells treated with Na3VO4 (0.3nM, 40mins).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Ezrin (phospho Y478) antibody (ab79256)All lanes : Anti-Ezrin (phospho Y478) antibody (ab79256) at 1/500 dilution
Lane 1 : K562 cell lysate treated with Na3VO4 (0.3nM,
40mins)
Lane 2 : K562 cell lysate treated with Na3VO4 (0.3nM,
40mins) with immunizing phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 69 kDa
Observed band size: 69 kDa
