Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PHACTR4 antibody [EP12543] (ab192882)

Key features and details

  • Rabbit monoclonal [EP12543] to PHACTR4
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-PHACTR4 antibody [EP12543]
    See all PHACTR4 primary antibodies

  • Description

    Rabbit monoclonal [EP12543] to PHACTR4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • SH-SY5Y, MCF7 and Y9 cell lysates; Human brain tissue; SH-SY5Y cell line.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Western blot - Anti-PHACTR4 antibody [EP12543] (ab192882)
    Western blot - Anti-PHACTR4 antibody [EP12543] (ab192882)
    Anti-PHACTR4 antibody [EP12543] (ab192882) at 1/10000 dilution + SH-SY5Y cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 78 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-PHACTR4 antibody [EP12543] (ab192882)
    Western blot - Anti-PHACTR4 antibody [EP12543] (ab192882)
    All lanes : Anti-PHACTR4 antibody [EP12543] (ab192882) at 1/1000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : Y79 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 78 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHACTR4 antibody [EP12543] (ab192882)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHACTR4 antibody [EP12543] (ab192882)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PHACTR4 with ab192882 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG as a secondary antibody. Counter stained with Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-PHACTR4 antibody [EP12543] (ab192882)
    Flow Cytometry - Anti-PHACTR4 antibody [EP12543] (ab192882)

    Flow cytometric analysis of 2% paraformaldehyde fixed SH-SY5Y cells labeling PHACTR4 using ab192882 at a 1/180 dilution (red). Goat anti rabbit IgG (FITC) was used as the secondary antibody at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (black). Unlabeled control cells without incubation with primary and secondary antibody (blue). 

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