Anti-PHACTR4 antibody [EP12543] - BSA and Azide free (ab269879)
Key features and details
- Rabbit monoclonal [EP12543] to PHACTR4 - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PHACTR4 antibody [EP12543] - BSA and Azide free
See all PHACTR4 primary antibodies -
Description
Rabbit monoclonal [EP12543] to PHACTR4 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SH-SY5Y, MCF7 and Y79 whole cell lysates. IHC-P: Human brain tissue. Flow cyt: SH-SY5Y cells.
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General notes
ab269879 is the carrier-free version of ab192882. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP12543 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHACTR4 antibody [EP12543] (ab269879)
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PHACTR4 with ab192882 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG as a secondary antibody. Counter stained with Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192882).
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Flow cytometric analysis of 2% paraformaldehyde fixed SH-SY5Y cells labeling PHACTR4 using ab192882 at a 1/180 dilution (red). Goat anti rabbit IgG (FITC) was used as the secondary antibody at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (black). Unlabeled control cells without incubation with primary and secondary antibody (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192882).