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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Binding Proteins

Anti-FHOD1 antibody (ab73443)

Anti-FHOD1 antibody (ab73443)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse polyclonal to FHOD1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-FHOD1 antibody
    See all FHOD1 primary antibodies
  • Description

    Mouse polyclonal to FHOD1
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    full-length human FHOD1 protein (NP_037373.2, 1 a.a. ~ 1164 a.a).

  • Positive control

    • K-562 cell lysate and FHOD1 transfected 293T cell lysate human spleen
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins

Images

  • Western blot - Anti-FHOD1 antibody (ab73443)
    Western blot - Anti-FHOD1 antibody (ab73443)
    All lanes : Anti-FHOD1 antibody (ab73443) at 1/500 dilution

    Lane 1 : FHOD1 transfected 293T cell lysate
    Lane 2 : non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : goat anti-mouse IgG(H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size: 127 kDa
    Observed band size: 145 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 80 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-FHOD1 antibody (ab73443)
    Western blot - Anti-FHOD1 antibody (ab73443)
    Anti-FHOD1 antibody (ab73443) at 1/500 dilution + K-562 cell lysate at 25 µg

    Secondary
    goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size: 127 kDa
    Observed band size: 150 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHOD1 antibody (ab73443)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHOD1 antibody (ab73443)
    Immunoperoxidase staining of formalin-fixed paraffin-embedded human spleen using ab73443, at 3 ug/ml of purified version of the antibody.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: ICC/IF, IHC-P

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    Applications: WB

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