Anti-FHOD1 antibody (ab73443)
Key features and details
- Mouse polyclonal to FHOD1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FHOD1 antibody
See all FHOD1 primary antibodies -
Description
Mouse polyclonal to FHOD1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
full-length human FHOD1 protein (NP_037373.2, 1 a.a. ~ 1164 a.a).
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Positive control
- K-562 cell lysate and FHOD1 transfected 293T cell lysate human spleen
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-FHOD1 antibody (ab73443) at 1/500 dilution
Lane 1 : FHOD1 transfected 293T cell lysate
Lane 2 : non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : goat anti-mouse IgG(H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 127 kDa
Observed band size: 145 kDa why is the actual band size different from the predicted?
Additional bands at: 80 kDa. We are unsure as to the identity of these extra bands.
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Anti-FHOD1 antibody (ab73443) at 1/500 dilution + K-562 cell lysate at 25 µg
Secondary
goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 127 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
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Immunoperoxidase staining of formalin-fixed paraffin-embedded human spleen using ab73443, at 3 ug/ml of purified version of the antibody.