Anti-Cofilin antibody (ab29038)
Key features and details
- Rabbit polyclonal to Cofilin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cofilin antibody
See all Cofilin primary antibodies -
Description
Rabbit polyclonal to Cofilin -
Host species
Rabbit -
Specificity
ab29038 recognises Cofilin 1. The immunogen sequence has only 3 amino acid differences from human Cofilin 2. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
A synthetic peptide (conjugated to KLH) corresponding to amino acid residues at the N terminus of human Cofilin 1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cofilin antibody (ab29038)Western blot of jurkat cells expressing cofilin 1. Blots were treated with (lanes 2, 3, 5 & 6) or without (lanes 1 & 4) lambda phosphatase for 1 hr at 37 degrees C. In lanes 3 & 6, phosphatase treatments were performed in the presence of phospho-cofilin 1 (Ser-3) peptide. Blots were probed with anti-phospho-Cofilin 1 (Ser-3) (lanes 1-3;) or anti-Cofilin 1 (lanes 4-6; ab29038). Phosphatase treatment removes Ser-3 phosphorylation of Cofilin, but this phosphatase activity is reduced in the presence of peptide.
