Anti-TAGLN/Transgelin antibody [EPR21215] (ab227566)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21215] to TAGLN/Transgelin
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-TAGLN/Transgelin antibody [EPR21215]
See all TAGLN/Transgelin primary antibodies -
Description
Rabbit monoclonal [EPR21215] to TAGLN/Transgelin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman -
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.
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Positive control
- WB: Human colon, Mouse colon and bladder tissue lysates gave a positive signal in western blot.
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General notes
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).
This product was previously labelled as TAGLN
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EPR21215 -
Isotype
IgG1 -
Research areas
Images
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All lanes : Anti-TAGLN/Transgelin antibody [EPR21215] (ab227566) at 1 µg/ml
Lane 1 : Human Skeletal Muscle tissue lysate
Lane 2 : Human Colon tissue lysate
Lane 3 : Mouse Skeletal Muscle tissue lysate
Lane 4 : Mouse Colon tissue lysate
Lane 5 : Mouse Bladder tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 23 kDaThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 40 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab213273 overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 secondary at 1/10000 dilution for 1 hour.
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