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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Binding Proteins

Anti-TAGLN/Transgelin antibody [EPR21215] (ab227566)

Price and availability

271 382 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-TAGLN/Transgelin antibody [EPR21215] (ab227566)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21215] to TAGLN/Transgelin
  • Suitable for: WB
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-TAGLN/Transgelin antibody [EPR21215]
    See all TAGLN/Transgelin primary antibodies
  • Description

    Rabbit monoclonal [EPR21215] to TAGLN/Transgelin
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Human colon, Mouse colon and bladder tissue lysates gave a positive signal in western blot.
  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

     This product was previously labelled as TAGLN

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21215
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Other
    • Cardiovascular
    • Heart
    • Contractility
    • Inotropics
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Smooth Muscle Cells

Images

  • Western blot - Anti-TAGLN/Transgelin antibody [EPR21215] (ab227566)
    Western blot - Anti-TAGLN/Transgelin antibody [EPR21215] (ab227566)
    All lanes : Anti-TAGLN/Transgelin antibody [EPR21215] (ab227566) at 1 µg/ml

    Lane 1 : Human Skeletal Muscle tissue lysate
    Lane 2 : Human Colon tissue lysate
    Lane 3 : Mouse Skeletal Muscle tissue lysate
    Lane 4 : Mouse Colon tissue lysate
    Lane 5 : Mouse Bladder tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 23 kDa



    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 40 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab213273 overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 secondary at 1/10000 dilution for 1 hour. 

  • Anti-TAGLN/Transgelin antibody [EPR21215] (ab227566)
    Anti-TAGLN/Transgelin antibody [EPR21215] (ab227566)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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