Antibodies, Proteins, Kits and Reagents for Life Science

Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (ab278686)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [4EB1T37T46-A5] to eIF4EBP1 (phospho T37 + T46)
Suitable for: WB, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5]
See all eIF4EBP1 primary antibodies
Description

Rabbit monoclonal [4EB1T37T46-A5] to eIF4EBP1 (phospho T37 + T46)
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide within Human eIF4EBP1 (phospho T37 + T46). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q13541

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Positive control
- WB: NIH/3T3 (treated with PDGF) cell extract; K562 cell extract. Flow cyt: C6 cells (treated with UV and TPA) ; Jurkat (treated with FBS) cells. \nIHC-P: Rat kidney.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
Flow Cyt	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Concentration information loading...
Purity

Protein A/G purified
Clonality

Monoclonal
Clone number

4EB1T37T46-A5
Isotype

IgG
Light chain type

kappa
Research areas

Flow Cytometry - Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (ab278686)

Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or treated with LY294002 + U0126 + wortmanmin (red) or with FBS (green) using 0.1 ug/mL of ab278686.
Western blot - Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (ab278686)

All lanes : Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (ab278686) at 0.001 µg/ml

Lane 1 : K562 cell extract untreated
Lane 2 : K562 cell extract treated with imatinib

Predicted band size: 13 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (ab278686)

Immunohistochemical staining of rat kidney cells including peptide blocking shows phospho-specificity for ab278686. Anti-rabbit secondary antibody staining (red) is punctate in the nuclei. Neither control peptide (A) nor non-phospho 4E-BP1 peptide (B) blocks staining but phospho 4E-BP1 (T37/T46) peptide efficiently blocks signal (C). No signal was observed when the primary antibody is omitted (D). The central object in the field is a glomerulus with surrounding tubules sectioned in various orientations.
Flow Cytometry - Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (ab278686)

Flow cytometric analysis of C6 cells untreated cells stained with secondary antibody as negative control (blue) or untreated (red) or treated with UV and TPA (green) and stained using ab278686 at 0.1µg/mL.
Flow Cytometry - Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (ab278686)

Peptide blocking flow cytometric analysis of Jurkat cells LY294002 plus wortmannin plus U0126 LWU) treated cells stained with secondary antibody as negative control (light blue) or treated with LWU (red) or treated with FBS (green) or LUW and blocked with phospho-peptide (black) or FBS and blocked with phospho peptide (gold) or LUW and blocked with non-phospho peptide (dark blue) or FBS and blocked with non-phospho peptide (purple) using ab278686 at 0.1µg/mL.
Western blot - Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (ab278686)

All lanes : Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (ab278686) at 0.001 µg/ml

Lane 1 : NIH3T3 cell extract untreated
Lane 2 : NIH3T3 cell extract treated with PDGF

Predicted band size: 13 kDa
Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (ab278686)

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