Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11107(2)] to SMYD3 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free
    See all SMYD3 primary antibodies

  • Description

    Rabbit monoclonal [EPR11107(2)] to SMYD3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type HAP1, HeLa, and MCF7 whole cell lysates. IHC-P: Human colonic carcinoma tissue. IP: HeLa cell lysate. Flow Cyt: HeLa cells.

  • General notes

    ab232626 is the carrier-free version of ab187149. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232626 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626)
    Western blot - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626)
    All lanes : Anti-SMYD3 antibody [EPR11107(2)] (ab187149) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : SMYD3 knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : MCF7 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 49 kDa
    Observed band size: 49 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187149).

    Lanes 1 - 4: Merged signal (red and green). Green - ab187149 observed at 49 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab187149 was shown to react with SMYD3 in HAP1 wild-type cells in Western blot. Loss of signal was observed when SMYD3 knockout sample was used. HAP1 wild-type and SMYD3 knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab187149 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue, labeling SMYD3 with ab187149 at 1/100 dilution. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187149).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626)
    Immunoprecipitation - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626)

    Immunoprecipitation analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate labeling SMYD3 using ab187149 at 1/50 dilution (Lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of HeLa lysate.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187149).

  • Flow Cytometry - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626)
    Flow Cytometry - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626)

    Immunohistochemical analysis of 2% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells, labeling SMYD3 with ab187149 at 1/30 dilution. Secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control was a Rabbit monoclonal IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187149).

  • Immunocytochemistry/ Immunofluorescence - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626)
    Immunocytochemistry/ Immunofluorescence - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells, labeling SMYD3 with ab187149 at 1/250 dilution (left image; green); secondary antibody was Goat anti rabbit IgG Alexa Fluor®488, and counterstained with Dapi (right image; blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187149).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626)

    Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue, labeling SMYD3 with ab187149 at 1/100 dilution. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187149).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626)
    Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626)

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