Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 09, 2021

Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11106(2)] to SMYD3 - N-terminal
Suitable for: IHC-P, WB, ICC/IF, IP, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-SMYD3 antibody [EPR11106(2)] - N-terminal
See all SMYD3 primary antibodies
Description

Rabbit monoclonal [EPR11106(2)] to SMYD3 - N-terminal
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, HAP1, 293, MCF-7 and T47-D cell lysates. IHC-P: Human testis and skeletal muscle tissue. ICC: HeLa cells. Flow Cyt: 293 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR11106(2)
Isotype

IgG
Research areas

Western blot - Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)

All lanes : Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : SMYD3 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : MCF7 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 49 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab183498 observed at 49 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab183498 was shown to recognize SMYD3 in wild-type HAP1 cells as signal was lost at the expected MW in SMYD3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SMYD3 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab183498 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)

All lanes : Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498) at 1/10000 dilution

Lane 1 : HeLa lysate
Lane 2 : 293 lysate
Lane 3 : T47-D lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 49 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle labeling SMYD3 at 1/100 dilution and HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)

Flow cytometric analysis of HeLa cells fixed in 20 °C acetone labeling SMYD3 at 1/500 dilution and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 (red). Counterstained with DAPI (blue)
Immunoprecipitation - Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)

Immunoprecipitation of HeLa cells labeling SMYD3 with ab183498 at 1/50 dilution and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.
Flow Cytometry - Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)

Flow cytometric analysis of 293 cells fixed in 2% paraformaldehyde labeling SMYD3 with ab183498 at 1/110 dilution and Goat anti rabbit IgG (FITC) at 1/150 dilution. Rabbit Monoclonal IgG was used as an isotype control.
Western blot - Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)

Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498) at 1/2000 dilution + MCF-7 lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 49 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)

Immunohistochemical analysis of paraffin-embedded Human testis labeling SMYD3 with ab183498 at 1/100 dilution and HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)

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