Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸ 10 µl 117 264 ₸

Order now and get it on Tuesday March 02, 2021

Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11107(2)] to SMYD3
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-SMYD3 antibody [EPR11107(2)]
See all SMYD3 primary antibodies
Description

Rabbit monoclonal [EPR11107(2)] to SMYD3
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Wild-type HAP1, MCF7, HeLa, HEK-293, T47-D and NIH/3T3 cell lysates; Mouse brain and Rat spleen tissue lysates. IHC-P: Human colonic carcinoma and mouse skeletal muscle tissues. ICC/IF: MCF7 and HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR11107(2)
Isotype

IgG
Research areas

Western blot - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

All lanes : Anti-SMYD3 antibody [EPR11107(2)] (ab187149) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : SMYD3 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : MCF7 whole cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 49 kDa
Observed band size: 49 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab187149 observed at 49 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab187149 was shown to react with SMYD3 in HAP1 wild-type cells in Western blot. Loss of signal was observed when SMYD3 knockout sample was used. HAP1 wild-type and SMYD3 knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween^®) before incubation with ab187149 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

All lanes : Anti-SMYD3 antibody [EPR11107(2)] (ab187149) at 1/20000 dilution

Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 4 : T47-D (human ductal breast epithelial tumor cell line) cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 49 kDa

Blocking and dilution buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells, labeling SMYD3 with ab187149 at 1/250 dilution (left image; green); secondary antibody was Goat anti rabbit IgG Alexa Fluor^®488, and counterstained with Dapi (right image; blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue, labeling SMYD3 with ab187149 at 1/100 dilution. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cytometry - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

Immunohistochemical analysis of 2% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells, labeling SMYD3 with ab187149 at 1/30 dilution. Secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control was a Rabbit monoclonal IgG.
Immunoprecipitation - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

Immunoprecipitation analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate labeling SMYD3 using ab187149 at 1/50 dilution (Lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of HeLa lysate.
Western blot - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

All lanes : Anti-SMYD3 antibody [EPR11107(2)] (ab187149) at 1/5000 dilution

Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat spleen tissue lysate
Lane 3 : NIH/3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 49 kDa

Blocking and dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue, labeling SMYD3 with ab187149 at 1/100 dilution. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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