Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562)
![Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562)](https://www.abcam.com/ps/products/278/ab278562/Images/ab278562-2-meks298h805ugml.jpg "Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MEK1S298-H8] to MEK1 (phospho S298)
- Suitable for: WB, Flow Cyt
- Reacts with: Rat, Human
Overview
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Product name
Anti-MEK1 (phospho S298) antibody [MEK1S298-H8]
See all MEK1 primary antibodies -
Description
Rabbit monoclonal [MEK1S298-H8] to MEK1 (phospho S298) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Synthetic peptide within Human MEK1 (phospho S298). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q02750 -
Positive control
- WB: C6 9treated with TPA) cell extract. Flow cyt: HeLa cells (treated with pervanadate); C6 cells (treated with MG132).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
MEK1S298-H8 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562)
Flow cytometric analysis of Hela cells secondary antibody only negative control (blue) or treated with imatinib (red) or treated with pervanadate (green) using ab278562 at 0.5 µg/mL.
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![Western blot - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562)](https://www.abcam.com/ps/products/278/ab278562/Images/ab278562-5-ab278562comparisonwb.jpg)
Western blot - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562)All lanes : Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562) at 0.005 µg/ml
Lane 1 : C6 cell extract treated with TPA
Lane 2 : C6 cell extract untreated with TPA
Predicted band size: 43 kDa
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![Flow Cytometry - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562)](https://www.abcam.com/ps/products/278/ab278562/Images/ab278562-4-20170810mekh8pepblock.jpg)
Flow Cytometry - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562)Peptide blocking flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or treated with MG132 (green) or treated and blocked with phospho peptide (orange) or treated and blocked with non-phospho peptide (purple) using ab278562 at 0.1 µg/mL. -
![Western blot - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562)](https://www.abcam.com/ps/products/278/ab278562/Images/ab278562-453787-AntiMEK1-phospho-S298-antibody-westernblot-C6.jpg)
Western blot - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562)All lanes : Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562) at 0.01 µg/ml
Lane 1 : C6 cell extract treated with TPA
Lane 2 : C6 cell extract untreated with TPA
Predicted band size: 43 kDa
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![Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562)](https://www.abcam.com/ps/products/278/ab278562/Images/ab278562-1-benefits-of-recombinant-antibodies.png)
Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562)