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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562)

Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [MEK1S298-H8] to MEK1 (phospho S298)
  • Suitable for: WB, Flow Cyt
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-MEK1 (phospho S298) antibody [MEK1S298-H8]
    See all MEK1 primary antibodies
  • Description

    Rabbit monoclonal [MEK1S298-H8] to MEK1 (phospho S298)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human MEK1 (phospho S298). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q02750

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: C6 9treated with TPA) cell extract. Flow cyt: HeLa cells (treated with pervanadate); C6 cells (treated with MG132).
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Clonality

    Monoclonal
  • Clone number

    MEK1S298-H8
  • Isotype

    IgG
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway

Images

  • Flow Cytometry - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562)
    Flow Cytometry - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562)

    Flow cytometric analysis of Hela cells secondary antibody only negative control (blue) or treated with imatinib (red) or treated with pervanadate (green) using ab278562 at 0.5 µg/mL.

  • Western blot - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562)
    Western blot - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562)
    All lanes : Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562) at 0.005 µg/ml

    Lane 1 : C6 cell extract treated with TPA
    Lane 2 : C6 cell extract untreated with TPA

    Predicted band size: 43 kDa

  • Flow Cytometry - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562)
    Flow Cytometry - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562)
    Peptide blocking flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or treated with MG132 (green) or treated and blocked with phospho peptide (orange) or treated and blocked with non-phospho peptide (purple) using ab278562 at 0.1 µg/mL.
  • Western blot - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562)
    Western blot - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562)
    All lanes : Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562) at 0.01 µg/ml

    Lane 1 : C6 cell extract treated with TPA
    Lane 2 : C6 cell extract untreated with TPA

    Predicted band size: 43 kDa

  • Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562)
    Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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