Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MLD antibody [EPR9681] - BSA and Azide free (ab249411)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9681] to MLD - BSA and Azide free
  • Suitable for: ICC/IF, WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-MLD antibody [EPR9681] - BSA and Azide free
    See all MLD primary antibodies

  • Description

    Rabbit monoclonal [EPR9681] to MLD - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 293T, T47D and HepG2 whole cell lysate (ab7900). IHC-P: Human breast carcinoma tissue.

  • General notes

    ab249411 is the carrier-free version of ab167169.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MLD antibody [EPR9681] - BSA and Azide free (ab249411)
    Western blot - Anti-MLD antibody [EPR9681] - BSA and Azide free (ab249411)
    All lanes : Anti-MLD antibody [EPR9681] (ab167169) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : DEGS1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 4 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    This data was developed using ab167169, the same antibody clone in a different buffer formulation.

    Lanes 1-4: Merged signal (red and green). Green - ab167169 observed at 38 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab167169 Anti-MLD antibody [EPR9681]  was shown to specifically react with MLD in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266481 (knockout cell lysate ab257918) was used. Wild-type and MLD knockout samples were subjected to SDS-PAGE. ab167169 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLD antibody [EPR9681] - BSA and Azide free (ab249411)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLD antibody [EPR9681] - BSA and Azide free (ab249411)
    This data was developed using ab167169, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling MLD with ab167169 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Western blot - Anti-MLD antibody [EPR9681] - BSA and Azide free (ab249411)
    Western blot - Anti-MLD antibody [EPR9681] - BSA and Azide free (ab249411)
    All lanes : Anti-MLD antibody [EPR9681] (ab167169) at 1/1000 dilution

    Lane 1 : 293T cell lysates
    Lane 2 : T47D cell lysates
    Lane 3 : HepG2 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 38 kDa



    This data was developed using ab167169, the same antibody clone in a different buffer formulation.

  • Anti-MLD antibody [EPR9681] - BSA and Azide free (ab249411)
    Anti-MLD antibody [EPR9681] - BSA and Azide free (ab249411)

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