Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BCMA antibody [EPR22457-260] - BSA and Azide free (ab254205)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22457-260] to BCMA - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-BCMA antibody [EPR22457-260] - BSA and Azide free
    See all BCMA primary antibodies

  • Description

    Rabbit monoclonal [EPR22457-260] to BCMA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: U266B1 whole cell lysate. ICC/IF: U266B1 cells. Flow cyt: U266B1 cells.

  • General notes

    Ab254205 is the carrier-free version of ab253242. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab254205 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-BCMA antibody [EPR22457-260] - BSA and Azide free (ab254205)
    Immunoprecipitation - Anti-BCMA antibody [EPR22457-260] - BSA and Azide free (ab254205)

    BCMA was immunoprecipitated from 0.35 mg of U266B1 (human multiple myeloma B lymphocyte cell line) whole cell lysate with ab253242 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab253242 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.

    Lane 1: U266B1 whole cell lysate 10 μg (Input).
    Lane 2: ab253242 IP in U266B1 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253242 in U266B1 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253242).

  • Flow Cytometry - Anti-BCMA antibody [EPR22457-260] - BSA and Azide free (ab254205)
    Flow Cytometry - Anti-BCMA antibody [EPR22457-260] - BSA and Azide free (ab254205)

    Flow cytometric analysis of  HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (left) or U266B1 (human multiple myeloma B lymphocyte cell line) (right) cells labeling BCMA with ab253242 at 1/60 dilution (red) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

    Negative control: HEK-293T cells (PMID: 18025285). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253242).

  • Immunocytochemistry/ Immunofluorescence - Anti-BCMA antibody [EPR22457-260] - BSA and Azide free (ab254205)
    Immunocytochemistry/ Immunofluorescence - Anti-BCMA antibody [EPR22457-260] - BSA and Azide free (ab254205)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U288B1 (human multiple myeloma B lymphocyte cell line) or HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling BCMA with ab253242 at 1/50 dilution, followed by a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
    Confocal image showing cytoplasmic and weak membranous staining in U266B1 cell line (PMID: 8527407).
    Negative control: HEK-293T cells (PMID: 18025285).
    The nuclear conter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253242).

  • Anti-BCMA antibody [EPR22457-260] - BSA and Azide free (ab254205)
    Anti-BCMA antibody [EPR22457-260] - BSA and Azide free (ab254205)

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