Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NMDAR1 antibody [EPR23397-66] - BSA and Azide free (ab274390)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23397-66] to NMDAR1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NMDAR1 antibody [EPR23397-66] - BSA and Azide free
    See all NMDAR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23397-66] to NMDAR1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse brain tissue lysate; Rat brain tissue lysate; Human brain tissue lysate. IHC-P: Human cerebrum tissue; Mouse cerebrum tissue; rat cerebrum tissue.

  • General notes

    ab274390 is the carrier-free version of ab274377.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NMDAR1 antibody [EPR23397-66] - BSA and Azide free (ab274390)
    Western blot - Anti-NMDAR1 antibody [EPR23397-66] - BSA and Azide free (ab274390)
    All lanes : Anti-NMDAR1 antibody [EPR23397-66] (ab274377) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : Mouse heart tissue lysate
    Lane 4 : Rat brain tissue lysate
    Lane 5 : Rat liver tissue lysate
    Lane 6 : Rat heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 105 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 10818139).

    Negative control: liver and heart (PMID: 10480904).

    Samples are non-boiled as boiling may cause protein aggregates.

    Exposure time: 5.5 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab274377)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR1 antibody [EPR23397-66] - BSA and Azide free (ab274390)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR1 antibody [EPR23397-66] - BSA and Azide free (ab274390)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling NMDAR1 with abab274377 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse cerebrum (PMID: 27391811). The section was incubated with ab274377 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab274377).

  • Western blot - Anti-NMDAR1 antibody [EPR23397-66] - BSA and Azide free (ab274390)
    Western blot - Anti-NMDAR1 antibody [EPR23397-66] - BSA and Azide free (ab274390)
    All lanes : Anti-NMDAR1 antibody [EPR23397-66] (ab274377) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 105 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Samples are non-boiled as boiling may cause protein aggregates.

    Exposure time: 15 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab274377)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR1 antibody [EPR23397-66] - BSA and Azide free (ab274390)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR1 antibody [EPR23397-66] - BSA and Azide free (ab274390)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling NMDAR1 with abab274377 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat cerebrum (PMID: 27391811). The section was incubated with ab274377 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab274377).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR1 antibody [EPR23397-66] - BSA and Azide free (ab274390)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR1 antibody [EPR23397-66] - BSA and Azide free (ab274390)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling NMDAR1 with abab274377 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: no staining on human liver (PMID: 10480904). The section was incubated with ab274377 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab274377).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR1 antibody [EPR23397-66] - BSA and Azide free (ab274390)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR1 antibody [EPR23397-66] - BSA and Azide free (ab274390)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling NMDAR1 with ab274377 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human cerebrum (PMID: 27391811). The section was incubated with ab274377 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab274377).

  • Anti-NMDAR1 antibody [EPR23397-66] - BSA and Azide free (ab274390)
    Anti-NMDAR1 antibody [EPR23397-66] - BSA and Azide free (ab274390)

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