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Neuroscience Neurotransmission Receptors / Channels Ligand-Gated Ion Channels NMDA Receptors

Anti-NMDAR2A antibody [EPR19694] (ab183939)

Price and availability

284 784 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-NMDAR2A antibody [EPR19694] (ab183939)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19694] to NMDAR2A
  • Suitable for: WB
  • Reacts with: Mouse, Rat

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Overview

  • Product name

    Anti-NMDAR2A antibody [EPR19694]
    See all NMDAR2A primary antibodies
  • Description

    Rabbit monoclonal [EPR19694] to NMDAR2A
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat hippocampus and brain lysates; His-tagged mouse NMDAR2A recombinant protein fragment (aa aa22-326).
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19694
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Ligand-Gated Ion Channels
    • NMDA Receptors

Images

  • Western blot - Anti-NMDAR2A antibody [EPR19694] (ab183939)
    Western blot - Anti-NMDAR2A antibody [EPR19694] (ab183939)
    All lanes : Anti-NMDAR2A antibody [EPR19694] (ab183939) at 1/1000 dilution

    Lane 1 : Mouse hippocampus lysate
    Lane 2 : Rat hippocampus lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Mouse heart lysate
    Lane 6 : Rat kidney lysate
    Lane 7 : Mouse spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 165 kDa
    Observed band size: 165 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 3 minutes; Lane 2: 30 seconds; Lane 3, 4, 5, 6 and 7: 3 minutes.

    NMDAR2A has been previously documented to be specifically expressed in neuron cells. (PMID: 12127670).

  • Western blot - Anti-NMDAR2A antibody [EPR19694] (ab183939)
    Western blot - Anti-NMDAR2A antibody [EPR19694] (ab183939)
    All lanes : Anti-NMDAR2A antibody [EPR19694] (ab183939) at 1/5000 dilution

    Lane 1 : His-tagged mouse NMDAR2A recombinant protein fragment (aa aa22-326)
    Lane 2 : His-tagged mouse NMDAR2B recombinant protein fragment (aa27-322)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 165 kDa
    Observed band size: 165 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 1 seconds; Lane 2: 3 minutes.

     

  • Anti-NMDAR2A antibody [EPR19694] (ab183939)
    Anti-NMDAR2A antibody [EPR19694] (ab183939)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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