Anti-NMDAR2A antibody [EPR19694] (ab183939)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19694] to NMDAR2A
- Suitable for: WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-NMDAR2A antibody [EPR19694]
See all NMDAR2A primary antibodies -
Description
Rabbit monoclonal [EPR19694] to NMDAR2A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse and rat hippocampus and brain lysates; His-tagged mouse NMDAR2A recombinant protein fragment (aa aa22-326).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19694 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-NMDAR2A antibody [EPR19694] (ab183939) at 1/1000 dilution
Lane 1 : Mouse hippocampus lysate
Lane 2 : Rat hippocampus lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Lane 5 : Mouse heart lysate
Lane 6 : Rat kidney lysate
Lane 7 : Mouse spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 165 kDa
Observed band size: 165 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 3 minutes; Lane 2: 30 seconds; Lane 3, 4, 5, 6 and 7: 3 minutes.
NMDAR2A has been previously documented to be specifically expressed in neuron cells. (PMID: 12127670).
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All lanes : Anti-NMDAR2A antibody [EPR19694] (ab183939) at 1/5000 dilution
Lane 1 : His-tagged mouse NMDAR2A recombinant protein fragment (aa aa22-326)
Lane 2 : His-tagged mouse NMDAR2B recombinant protein fragment (aa27-322)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 165 kDa
Observed band size: 165 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 1 seconds; Lane 2: 3 minutes.
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