Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TPMT antibody [EPR10820(B)] (ab155284)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10820(B)] to TPMT
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-TPMT antibody [EPR10820(B)]
    See all TPMT primary antibodies

  • Description

    Rabbit monoclonal [EPR10820(B)] to TPMT

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    A synthetic peptide, corresponding to residues in Human TPMT (UniProt: P51580).

  • Positive control

    • HAP1, K562, TF1, Jurkat and HepG2 cell lysates; Human brain and kidney tissue; Permeabilized K562 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TPMT antibody [EPR10820(B)] (ab155284)
    Western blot - Anti-TPMT antibody [EPR10820(B)] (ab155284)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: TPMT knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HepG2 whole cell lysate (20 µg)
    Lane 4: Jurkat whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab155284 observed at 28 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab155284 was shown to recognize TPMT in wild-type HAP1 cells as signal was lost at the expected MW in TPMT knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TPMT knockout samples were subjected to SDS-PAGE. Ab155284 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 2 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-TPMT antibody [EPR10820(B)] (ab155284)
    Western blot - Anti-TPMT antibody [EPR10820(B)] (ab155284)
    All lanes : Anti-TPMT antibody [EPR10820(B)] (ab155284) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : TF1 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 28 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling TPMT with ab155284 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TPMT with ab155284 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-TPMT antibody [EPR10820(B)] (ab155284)
    Flow Cytometry - Anti-TPMT antibody [EPR10820(B)] (ab155284)
    Flow cytometric analysis of permeabilized K562 cells labeling TPMT with ab155284 at 1/100 dilution (red) compared with a rabbit IgG (negative) (green).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)

    Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab155284 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)

    Immunohistochemical analysis of paraffin embedded Human gastric adenocarcinoma tissue using ab155284 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)

    Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab155284 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-TPMT antibody [EPR10820(B)] (ab155284)
    Anti-TPMT antibody [EPR10820(B)] (ab155284)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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