Anti-TPMT antibody (ab236648)
Images
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All lanes : Anti-TPMT antibody (ab236648) at 1/1000 dilution
Lane 1 : EC109 whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/50000 dilution
Predicted band size: 28 kDa
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Paraffin-embedded human kidney tissue stained for TPMT using ab236648 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human brain tissue stained for TPMT using ab236648 at 1/100 dilution in immunohistochemical analysis.
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TPMT was immunoprecipitated from 500 µg K562 (human liver chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab236648 at 1/200 dilution.
Lane 1: Rabbit control IgG IP in K562 whole cell lysate.
Lane 2: ab236648 IP in K562 whole cell lysate.
Lane 3: K562 whole cell lysate 20 µg (Input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.