Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TPMT antibody [EPR10821(B)] (ab166909)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10821(B)] to TPMT
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-TPMT antibody [EPR10821(B)]
    See all TPMT primary antibodies

  • Description

    Rabbit monoclonal [EPR10821(B)] to TPMT

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues in Human TPMT (UniProt P51580).

  • Positive control

    • K562, HepG2, Jurkat, TF-1 cell lysate. K562 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-TPMT antibody [EPR10821(B)] (ab166909)
    Western blot - Anti-TPMT antibody [EPR10821(B)] (ab166909)
    All lanes : Anti-TPMT antibody [EPR10821(B)] (ab166909) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : TPMT knockout HAP1 whole cell lysate
    Lane 3 : HepG2 whole cell lysate
    Lane 4 : K562 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 28 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab166909 observed at 28 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab166909 was shown to recognize TPMT in wild-type HAP1 cells as signal was lost at the expected MW in TPMT knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TPMT knockout samples were subjected to SDS-PAGE. Ab166909 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at  1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-TPMT antibody [EPR10821(B)] (ab166909)
    Western blot - Anti-TPMT antibody [EPR10821(B)] (ab166909)
    All lanes : Anti-TPMT antibody [EPR10821(B)] (ab166909) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : TF-1 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 28 kDa

  • Flow Cytometry - Anti-TPMT antibody [EPR10821(B)] (ab166909)
    Flow Cytometry - Anti-TPMT antibody [EPR10821(B)] (ab166909)
    Flow cytometric analysis of permeabilized K562 cells using ab166909 at a 1/100 dilution (red) or a rabbit IgG (negative) (green).
  • Anti-TPMT antibody [EPR10821(B)] (ab166909)
    Anti-TPMT antibody [EPR10821(B)] (ab166909)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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