Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-TPMT antibody [EPR10821(B)] (ab166909)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10821(B)] to TPMT
Suitable for: WB, Flow Cyt
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-TPMT antibody [EPR10821(B)]
See all TPMT primary antibodies
Description

Rabbit monoclonal [EPR10821(B)] to TPMT
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to residues in Human TPMT (UniProt P51580).
Positive control
- K562, HepG2, Jurkat, TF-1 cell lysate. K562 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR10821(B)
Isotype

IgG
Research areas

Western blot - Anti-TPMT antibody [EPR10821(B)] (ab166909)

All lanes : Anti-TPMT antibody [EPR10821(B)] (ab166909) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : TPMT knockout HAP1 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : K562 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 28 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab166909 observed at 28 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab166909 was shown to recognize TPMT in wild-type HAP1 cells as signal was lost at the expected MW in TPMT knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TPMT knockout samples were subjected to SDS-PAGE. Ab166909 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-TPMT antibody [EPR10821(B)] (ab166909)

All lanes : Anti-TPMT antibody [EPR10821(B)] (ab166909) at 1/1000 dilution

Lane 1 : K562 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : TF-1 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 28 kDa
Flow Cytometry - Anti-TPMT antibody [EPR10821(B)] (ab166909)

Flow cytometric analysis of permeabilized K562 cells using ab166909 at a 1/100 dilution (red) or a rabbit IgG (negative) (green).
Anti-TPMT antibody [EPR10821(B)] (ab166909)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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