Detected using chemiluminescence.

Immunocytochemistry/Immunofluorescence analysis of HepG2 labelling NQO1 with ab2346 at 5 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor^® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.

Negative control: Unimmunized goat IgG (5 µg/ml) followed by an Alexa Fluor^® 488-conjugated secondary antibody (2 µg/ml).

Detected using chemiluminescence.

Immunocytochemistry/Immunofluorescence analysis of U251 labelling NQO1 with ab2346 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor^® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.

Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor^® 488-conjugated secondary antibody (2 µg/ml).

Detected using chemiluminescence.

ICC/IF image of ab2346 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2346, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor^® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.