Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Rat monoclonal [M18/2] to CD18
Suitable for: ICC, Flow Cyt
Reacts with: Mouse
Isotype: IgG2a

Product name

Anti-CD18 antibody [M18/2]
See all CD18 primary antibodies
Description

Rat monoclonal [M18/2] to CD18
Host species

Rat
Tested applications

Suitable for: ICC, Flow Cytmore details
Species reactivity

Reacts with: Mouse
Does not react with: Dog
Immunogen

Full length protein corresponding to Mouse CD18. Murine cytotoxic T cell glycoproteins
Positive control
- Flow Cyt: Mouse splenocytes and myeloid cells.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Protein A purified
Purification notes

> 95% (by SDS-PAGE)
Clonality

Monoclonal
Clone number

M18/2
Isotype

IgG2a
Research areas

Flow Cytometry - Anti-CD18 antibody [M18/2] (ab119830)

Flow cytometry analysis of mouse splenocytes labeling CD18 with ab119830 at 16 μg/mL.
Immunocytochemistry - Anti-CD18 antibody [M18/2] (ab119830) This image is courtesy of an anonymous abreview.

ICC/IF image of CD18 staining on RAW264.7 cells using ab119830 (1:300). The cells were fixed using formaldehyde and later permeabilized using 0.2% Triton X-100. The cells were blocked using 10% goat serum for 1 hour at 20°C. ab119830 was diluted to 1:300 and incubated with the cells for 1 hour at 20°C. The secondary antibody used was Anti-rat goat polyclonal conjugated to Alexa Fluor 647 (1:350).
See Abreview
Flow Cytometry - Anti-CD18 antibody [M18/2] (ab119830)

Flow Cytometry analysis of mouse myeloid cells labeling CD18 with ab119830 at 16 μg/mL (red-filled).

Mouse myeloid cells unstained by primary antibody (DAR APC, black-dashed).

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