Anti-mouse IgG fab antibody [RMG05] (ab197774)
![Anti-mouse IgG fab antibody [RMG05] (ab197774)](https://www.abcam.com/ps/products/197/ab197774/Images/ab197774-242433-anti-mouse-igg-fab-antibody-rmg05-elisa.jpg "Anti-mouse IgG fab antibody [RMG05] (ab197774)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Goat monoclonal [RMG05] to mouse IgG fab
- Suitable for: ELISA
- Reacts with: Mouse
Overview
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Product name
Anti-mouse IgG fab antibody [RMG05] -
Description
Goat monoclonal [RMG05] to mouse IgG fab -
Host species
Goat -
Specificity
ab197774 reacts to the Fab region of Mouse IgG, including IgG1, IgG2a, IgG2b, and IgG3. No cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, or rabbit IgG. -
Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Full length protein corresponding to mouse IgG fab.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RMG05 -
Isotype
IgG1
Images
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ELISA - Anti-mouse IgG fab antibody [RMG05] (ab197774)
A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of ab197774 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
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![ELISA - Anti-mouse IgG fab antibody [RMG05] (ab197774)](https://www.abcam.com/ps/products/197/ab197774/Images/ab197774-242432-anti-mouse-igg-fab-antibody-rmg05-elisa.jpg)
ELISA - Anti-mouse IgG fab antibody [RMG05] (ab197774)ELISA of mouse immunoglobulins shows ab197774 reacted to the Fab region of mouse IgG1, IgG2a, and IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG. The plate was coated with 50 ng/well of different immunoglobulins. 500 ng/mL, 200 ng/mL, or 50 ng/mL of ab197774 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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![Anti-mouse IgG fab antibody [RMG05] (ab197774)](https://www.abcam.com/ps/products/197/ab197774/Images/ab197774-3-benefits-of-recombinant-antibodies.png)
Anti-mouse IgG fab antibody [RMG05] (ab197774)