Antibodies, Proteins, Kits and Reagents for Life Science

Anti-CD18 (phospho S745) antibody [EP1288Y] - BSA and Azide free (ab232579)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1288Y] to CD18 (phospho S745) - BSA and Azide free
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-CD18 (phospho S745) antibody [EP1288Y] - BSA and Azide free
See all CD18 primary antibodies
Description

Rabbit monoclonal [EP1288Y] to CD18 (phospho S745) - BSA and Azide free
Host species

Rabbit
Specificity

This antibody only detects CD18 phosphorylated on Serine 745.
Tested applications

Suitable for: WB, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Jurkat cell lysate.
General notes
Ab232579 is the carrier-free version of ab52920. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab232579 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1288Y
Isotype

IgG
Research areas

Immunoprecipitation - Anti-CD18 (phospho S745) antibody [EP1288Y] - BSA and Azide free (ab232579)

ab52920 (purified) at 1:50 dilution (2µg) immunoprecipitating CD18 in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab52920 & Jurkat whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52920 in Jurkat whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52920).
Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] - BSA and Azide free (ab232579)

All lanes : Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920) at 1/500 dilution (unpurified)

Lane 1 : Un-treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : AP-treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 85 kDa

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52920).
Anti-CD18 (phospho S745) antibody [EP1288Y] - BSA and Azide free (ab232579)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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