Anti-CD18 antibody [MEM-48] - Low endotoxin, Azide free (ab46700)
![Anti-CD18 antibody [MEM-48] - Low endotoxin, Azide free (ab46700)](https://www.abcam.com/ps/products/46/ab46700/Images/ab46700-58934-anti-cd18-antibody-mem-48-flow-cytometry.jpg "Anti-CD18 antibody [MEM-48] - Low endotoxin, Azide free (ab46700)")
Key features and details
- Mouse monoclonal [MEM-48] to CD18 - Low endotoxin, Azide free
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD18 antibody [MEM-48] - Low endotoxin, Azide free
See all CD18 primary antibodies -
Description
Mouse monoclonal [MEM-48] to CD18 - Low endotoxin, Azide free -
Host species
Mouse -
Specificity
The antibody MEM-48 recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin ß2 subunit; ß2 integrin). -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Leucocytes of a patient suffering from a LGL-type leukaemia.
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Positive control
- WB: Kg-1a human leukemia cell lysate
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General notes
Preserved by filter sterilization
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from TCS. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
MEM-48 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-CD18 antibody [MEM-48] - Low endotoxin, Azide free (ab46700)
Overlay histogram showing peripheral blood lymphocytes stained with ab46700 (red line). The cells were incubated with the antibody (ab46700, 1µg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/200 dilution for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.
