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Signal Transduction Metabolism Plasma Membrane

Human TJP1 (ZO1 tight junction protein) knockout HeLa cell lysate (ab257744)

Price and availability

318 288 ₸

Availability

Order now and get it on Friday March 05, 2021

Human TJP1 (ZO1 tight junction protein) knockout HeLa cell lysate (ab257744)
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Overview

  • Product name

    Human TJP1 (ZO1 tight junction protein) knockout HeLa cell lysate
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HeLa
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 2.
  • Passage number

  • Knockout validation

    Sanger Sequencing
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab260337 - Human TJP1 knockout HeLa cell lysate 1 x 100µg
    ab255552 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Plasma Membrane
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female
  • STR Analysis

    Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10

Target

  • Function

    The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.
  • Tissue specificity

    The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
  • Sequence similarities

    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
    Contains 1 ZU5 domain.
  • Domain

    The second PDZ domain mediates interaction with GJA12.
  • Post-translational
    modifications

    Phosphorylated. Dephosphorylated by PTPRJ.
  • Cellular localization

    Cell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.
  • Target information above from: UniProt accession Q07157 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Tight junction protein 1
    • Tight junction protein ZO-1
    • Tight junction protein ZO1
    • TJP1
    • zo-1
    • Zo1
    • ZO1_HUMAN
    • Zona occludens 1
    • Zona occludens 1 protein
    • Zona occludens protein 1
    • Zonula occludens 1 protein
    • Zonula occludens protein 1
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab260337 - Human TJP1 knockout HeLa cell lysate 1 x 100µg
    ab255552 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Plasma Membrane
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female
  • STR Analysis

    Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10

Images

  • Sanger Sequencing - Human TJP1 knockout HeLa cell lysate (ab257744)
    Sanger Sequencing - Human TJP1 knockout HeLa cell lysate (ab257744)
    Homozygous: 1 bp deletion in exon 2

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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