Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Wednesday March 03, 2021

Anti-SHCBP1 antibody [EPR18843] (ab184467)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18843] to SHCBP1
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-SHCBP1 antibody [EPR18843]
See all SHCBP1 primary antibodies
Description

Rabbit monoclonal [EPR18843] to SHCBP1
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Mouse

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: NIH/3T3 treated with 0.5% FBS for 48 hours then changed to 20% FBS for 28 hours whole cell lysate; Mouse embryo lysate; P0 mouse brain lysate; Rat embryo lysate; P0 rat brain lysate; Mouse thymus and lung lysates; Human fetal heart and fetal kidney lysates; HeLa, A431, Jurkat, Neuro-2a, C6, PC-12, RAW 264.7 and NIH/3T3 whole cell lysates. IP: Mouse thymus whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Mouse
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18843
Isotype

IgG
Research areas

Western blot - Anti-SHCBP1 antibody [EPR18843] (ab184467)

All lanes : Anti-SHCBP1 antibody [EPR18843] (ab184467) at 1/5000 dilution

Lane 1 : NIH/3T3 (Mouse embryonic fibroblast cell line) treated with 0.5% FBS for 48 hours whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) treated with 0.5% FBS for 48 hours then changed to 20% FBS for 28 hours whole cell lysate
Lane 3 : Mouse embryo lysate
Lane 4 : P0 mouse brain lysate
Lane 5 : Rat embryo lysate
Lane 6 : P0 rat brain lysate
Lane 7 : Mouse liver lysate
Lane 8 : Mouse thymus lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 76 kDa
Observed band size: 76 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1, 2, 3, 4, 5 and 6: 3 minutes; Lane 7 and 8: 30 seconds.

SHCBP1 plays important roles in embryonic development, cytokinesis, neuronal development, tumorigenesis and T cell development. It is highly expressed in thymus but absent in liver. It can be induced in serum-stimulated NIH/3T3 cells. (PMID: 10086341 and 25153088).
Western blot - Anti-SHCBP1 antibody [EPR18843] (ab184467)

All lanes : Anti-SHCBP1 antibody [EPR18843] (ab184467) at 1/1000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 76 kDa
Observed band size: 76 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1: 30 seconds; Lane 2: 8 seconds.
Western blot - Anti-SHCBP1 antibody [EPR18843] (ab184467)

All lanes : Anti-SHCBP1 antibody [EPR18843] (ab184467) at 1/5000 dilution

Lane 1 : Mouse lung lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 5 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 76 kDa
Observed band size: 76 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposuretimes: Lane 1, 2, 3 and 4: 30 seconds; Lane 5: 8 seconds.
Western blot - Anti-SHCBP1 antibody [EPR18843] (ab184467)

All lanes : Anti-SHCBP1 antibody [EPR18843] (ab184467) at 1/1000 dilution

Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 3 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 76 kDa
Observed band size: 76 kDa

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-SHCBP1 antibody [EPR18843] (ab184467)

SHCBP1 was immunoprecipitated from 0.35 mg of Mouse thymus whole cell lysate with ab184467 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184467 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Mouse thymus whole cell lysate, 10µg (Input).

Lane 2: ab184467 IP in Mouse thymus whole cell lysate.

Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab184467 in Mouse thymus whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 seconds.
Anti-SHCBP1 antibody [EPR18843] (ab184467)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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